Ability of monoclonal anti-D antibodies to promote the binding of red cells to lymphocytes, granulocytes and monocytes

Vox Sanguinis
A H MerryN C Hughes-Jones

Abstract

Red cells sensitized with IgG1 or IgG3 monoclonal anti-D antibodies were used in rosette assays with human lymphocytes, monocytes and granulocytes. With all three cell types. IgG3 antibodies promoted a greater degree of rosette formation than IgG1 antibodies. Monocytes required a minimum of about 0.5 x 10(3) IgG3 molecules per red cell for rosette formation, and granulocytes and lymphocytes required around 1 x 10(4) IgG3 molecules per red cell. Approximately 80% of monocytes and granulocytes and 10% of lymphocytes were capable of rosette formation. These results are consistent with differences in the number and affinity of Fc receptors on different leucocytes. When compared with previous data these results suggest that binding of monocytes to monoclonal anti-D sensitized red cells is very similar to that of red cells sensitized with polyclonal antisera. Lymphocytes and granulocytes, however, appear to bind less well to red cells sensitized with certain monoclonal antibodies than with polyclonal antibodies. These findings may be of relevance to the prophylactic use of monoclonal anti-D antibodies.

References

Dec 1, 1979·British Journal of Haematology·S BarrettG Garratty
Aug 1, 1977·British Journal of Haematology·F W VerheugtC P Engelfriet
Jan 1, 1989·Clinical and Laboratory Haematology·A G HadleyB Zupanska
Jun 1, 1987·British Journal of Haematology·S S ArmstrongM Contreras
Mar 1, 1986·Molecular Immunology·J M WoofD R Burton
Mar 1, 1985·Molecular Immunology·D R Burton
Apr 15, 1985·American Journal of Obstetrics and Gynecology·J ParinaudG Pontonnier
Feb 19, 1983·Lancet·D H CrawfordE R Huehns
Jan 1, 1980·International Archives of Allergy and Applied Immunology·M D Alexander
Sep 1, 1986·Immunology Today·C L Anderson, R J Loone

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Citations

Mar 1, 1995·Transfusion Medicine·I M BromilowJ K Duguid
Aug 14, 2012·American Journal of Hematology·Sol SchulmanDavid J Kuter
Oct 1, 1995·Transfusion Medicine Reviews·A Fletcher, A Thomson
Mar 23, 2010·Transfusion Medicine Reviews·Eleftherios C Vamvakas, Morris A Blajchman
Jan 1, 1990·Clinical and Laboratory Haematology·B Zupańska

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