Ability of sulfated glycoconjugates and disulfide-reductants to release bovine epididymal sperm bound to the oviductal epithelium in vitro

Theriogenology
R GualtieriR Talevi

Abstract

In Bos taurus, at ejaculation, epididymal sperm acquire a number of proteins secreted in the seminal plasma that increase their ability to interact with the female reproductive tract. Sperm-oviduct interaction comprises a transient sperm adhesion to the isthmus, the lower portion of the oviduct, followed by sperm release around ovulation. Oviductal fluid molecules, such as sulfated glycoconjugates and disulfide-reductants, are able to release bovine ejaculated sperm bound to the oviductal epithelium in vitro through the reduction of sperm surface protein disulfides to sulfhydryls. To understand whether the sperm molecules sensitive to releasing signals are already exposed on the surface of epididymal sperm, we studied the ability of cauda epididymal sperm to adhere to the oviductal epithelium and to be released by sulfated glycoconjugates and the disulfide-reductant penicillamine. Surface protein sulfhydryls in cauda epididymal sperm were analyzed in the initial suspension, in sperm bound to the in vitro-cultured oviductal epithelium, and in released sperm. Results showed that epididymal sperm are able to bind the oviductal epithelium in vitro, although at a lower extent than frozen-thawed ejaculated sperm; the interaction is m...Continue Reading

References

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Dec 24, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Roberto GualtieriRiccardo Talevi
Jan 19, 2007·Biology of Reproduction·Riccardo TaleviRoberto Gualtieri
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Apr 23, 2009·Reproduction, Fertility, and Development·Hanae Pons-RejrajiPierre Leclerc
May 15, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Roberto GualtieriRiccardo Talevi
Aug 18, 2009·Theriogenology·R Talevi, R Gualtieri

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Citations

Sep 6, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Sergio GhersevichCarlos Zumoffen
Nov 26, 2010·Journal of Assisted Reproduction and Genetics·Brian Dale, Louis DeFelice
Jan 13, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Bart LeemansAnn Van Soom
Dec 26, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·T LeahyS P de Graaf
Mar 5, 2016·Reproduction in Domestic Animals = Zuchthygiene·M S FerrerM Wilkerson
Oct 5, 2010·Molecular Reproduction and Development·William V Holt, Alireza Fazeli
Jun 15, 2012·Reproduction in Domestic Animals = Zuchthygiene·I MondéjarM Avilés
May 19, 2018·Reproduction, Fertility, and Development·A LyonsS Fair

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