Ability to activate oocytes and chromosome integrity of mouse spermatozoa preserved in EGTA Tris-HCl buffered solution supplemented with antioxidants

Theriogenology
H Kusakabe, Yujiroh Kamiguchi

Abstract

Potential methods for cryopreservation of mouse spermatozoa are freeze-drying, desiccation, and suspension in EGTA Tris-HCl buffered solution (ETBS: 50 mM NaCl, 50 mM EGTA, and 10 mM Tris-HCl). To determine the duration that mouse spermatozoa suspended in ETBS-based solutions could retain their normal characteristics without freezing, spermatozoa collected from the cauda epididymis were suspended in ETBS or in ETBS supplemented with the antioxidants, dimethyl sulfoxide (DMSO), or DL-alpha-tocopherol acetate (Vitamin E acetate; VEA) diluted in DMSO, then held at ambient temperature (22-24 degrees C) for up to 9 days. When oocytes were injected with spermatozoa preserved in ETBS alone, activation rates of oocytes and chromosome integrity at the first cleavage metaphase decreased at 1 day (P < 0.001) and 2-4 days (P < 0.01) following treatment. When oocytes were injected with spermatozoa preserved in ETBS supplemented with DMSO or VEA/DMSO, chromosome integrity did not decrease significantly (through 9 days of preservation). Although DMSO maintained sperm chromosome integrity more effectively than VEA/DMSO up to 2-4 days (91 and 67%, normal karyotypes in DMSO and VEA/DMSO, respectively), VEA/DMSO helped to maintain the ability of ...Continue Reading

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Citations

Nov 5, 2011·The Journal of Reproduction and Development·Shinichi HochiMasumi Hirabayashi
Jun 6, 2009·Journal of Medical Primatology·Stuart A MeyersJames W Overstreet
Feb 15, 2005·Biochemical and Biophysical Research Communications·Qi-Cai LiuFang-Zhen Sun
Oct 20, 2004·Mutation Research·Hirokazu Kusakabe, Yujiroh Kamiguchi
May 24, 2019·The Journal of Reproduction and Development·Daiyu ItoTeruhiko Wakayama
Jun 1, 2011·Reproductive Medicine and Biology·Hirokazu Kusakabe

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