Ablation of NMDA receptors enhances the excitability of hippocampal CA3 neurons.

PloS One
Fumiaki FukushimaMasayoshi Mishina

Abstract

Synchronized discharges in the hippocampal CA3 recurrent network are supposed to underlie network oscillations, memory formation and seizure generation. In the hippocampal CA3 network, NMDA receptors are abundant at the recurrent synapses but scarce at the mossy fiber synapses. We generated mutant mice in which NMDA receptors were abolished in hippocampal CA3 pyramidal neurons by postnatal day 14. The histological and cytological organizations of the hippocampal CA3 region were indistinguishable between control and mutant mice. We found that mutant mice lacking NMDA receptors selectively in CA3 pyramidal neurons became more susceptible to kainate-induced seizures. Consistently, mutant mice showed characteristic large EEG spikes associated with multiple unit activities (MUA), suggesting enhanced synchronous firing of CA3 neurons. The electrophysiological balance between fast excitatory and inhibitory synaptic transmission was comparable between control and mutant pyramidal neurons in the hippocampal CA3 region, while the NMDA receptor-slow AHP coupling was diminished in the mutant neurons. In the adult brain, inducible ablation of NMDA receptors in the hippocampal CA3 region by the viral expression vector for Cre recombinase als...Continue Reading

References

Mar 23, 1992·FEBS Letters·M YamazakiM Mishina
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H T ClineM Constantine-Paton
Jun 1, 1994·Current Opinion in Neurobiology·M F Bear, R C Malenka
Jun 1, 1993·The Japanese Journal of Psychiatry and Neurology·K MiuraM Kito
Jan 7, 1993·Nature·T V Bliss, G L Collingridge
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·M StockerP Pedarzani
Sep 18, 1999·Science·R C Malenka, R A Nicoll
Sep 21, 2000·Epilepsy Research·M Quigg
Jun 1, 2002·Science·Kazu NakazawaSusumu Tonegawa
Jun 8, 2002·Biochemical and Biophysical Research Communications·Tomonori TakeuchiMasayoshi Mishina
Apr 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masahiro FukayaMasahiko Watanabe
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Natasa SavićR Anne McKinney
May 13, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Richard G M Morris
Jun 26, 2003·The European Journal of Neuroscience·Taisuke MiyazakiMasahiko Watanabe
Oct 31, 2003·Trends in Neurosciences·J Josh Lawrence, Chris J McBain
Nov 19, 2003·The European Journal of Neuroscience·Cynthia D GalvanJohn W Swann
Feb 25, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tomonori TakeuchiMasayoshi Mishina
Apr 26, 2005·Neuron·Masayoshi MurakamiKensaku Mori
Apr 27, 2005·Nature Neuroscience·Thu Jennifer Ngo-AnhJohn P Adelman

❮ Previous
Next ❯

Citations

Sep 2, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lindsay M De BiaseDwight E Bergles
Sep 17, 2015·Brain : a Journal of Neurology·Sukhvir WrightA Louise Upton
Nov 23, 2011·Genes, Brain, and Behavior·M E HawsC M Powell

❮ Previous
Next ❯

Methods Mentioned

BETA
phosphotransferase
PCR
electrophoresis
dot-blot
X-ray
MDA

Software Mentioned

IMARIS
LabVIEW
Bitplane
FilamentTracer
IGOR ( Wave matics )
IP Lab

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.