Abnormal Ca2+ signalling in vascular endothelial cells from spontaneously hypertensive rats: role of free radicals

Journal of Hypertension
L PoganR Sauvé

Abstract

To test the hypothesis that the Ca2+ signal transduction process in endothelial cells from genetically hypertensive rats (SHR) is affected by an overproduction of free radicals. The Ca2+ response to the inositol 1,4,5-triphosphate (IP3) mobilizing agonist, ATP, was measured using the fluorescent probe, fura-2, in endothelial cells from Sprague-Dawley rats, and in young and age-matched genetically hypertensive rats (SHR). The effect of free radicals and reducing agents on the intracellular release of Ca2+ and IP3productionwas determined in resting and ATP-stimulated cells. Experiments were also performed to compare the level of expression and enzymatic activity of catalase and superoxide dismutase (SOD) in endothelial cells from SHR and Sprague-Dawley rats. The exposure of aortic endothelial cells from Sprague-Dawley rats to the free-radical generating system, hypoxanthine + xanthine oxidase (HX/XO), caused a time- and concentration-dependent inhibition of the ATP-induced Ca2+ response. A similar HX/XO-dependent inhibition was also observed in Sprague-Dawley cells stimulated with the endoplasmic reticulum Ca2+-ATPase inhibitor, thapsigargin. Incubation with the antioxidative enzymes, catalase and SOD, had no effect on the ATP-in...Continue Reading

References

Jan 1, 1992·Journal of Cardiovascular Pharmacology·D Fu-XiangD Diederich
Mar 1, 1989·The American Journal of Physiology·A K Grover, S E Samson
Mar 3, 1994·Nature·M FeelischS Moncada
Nov 1, 1995·Free Radical Biology & Medicine·S J Elliott, S K Koliwad
Jun 5, 1996·Cancer Letters·G GünerU Açikel
Apr 29, 1998·The Journal of Clinical Investigation·F CosentinoT F Lüscher
Aug 6, 1999·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·A A De Artinano, V L Gonzalez
Oct 16, 1999·Hypertension·M McIntyreA F Dominiczak
Oct 16, 1999·The Journal of Physiology·P BissonnetteJ Y Lapointe
Mar 4, 2000·Journal of Pharmaceutical and Biomedical Analysis·V BrovkovychT Malinski

❮ Previous
Next ❯

Citations

Nov 30, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Peter Johnson
Sep 12, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Nauman AzizAnwarul Hassan Gilani
Jul 19, 2006·Canadian Journal of Physiology and Pharmacology·Lingyun Wu
Apr 20, 2004·Journal of Cellular and Molecular Medicine·A ManeaMonica Raicu
Nov 23, 2006·Brain Research Bulletin·Leonardo Máximo CardosoEduardo Colombari
Apr 11, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Jiaxuan ZhuJulian H Lombard
Jul 18, 2002·Journal of Pineal Research·L PoganR Sauvé
Sep 23, 2006·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Frank A DeLanoGeert W Schmid-Schönbein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.