Abnormal change of p53 gene in gastric and precancerous lesions and APC gene deletion in gastric carcinoma and near tissues

Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao
Y HaoW Qian

Abstract

p53 gene mutation (exon4, 5, 6, 7, 8 and intron6) in gastric cancer and precancerous lesions and p53 gene (exon4 and ontron6), APC gene deletion in gastric carcinomas were studied by PCR/SSCP and PCR/RFLP. Results showed mutation rate of p53 in metaplasia, dysplasia and gastric carcinoma was 37.5% (3/8), 42.17% (8/19), 53.33 (16/30) respectively. There was significant difference among groups of metaplasia, dysplasia, cancer and normal controls. No exon8 mutation was found in metaplasia and dysplasia, but 4 cases were found to have exon8 mutation in cancer group. It is suggested that exon8 mutation occurs at the late stage of gastric cancer, but exon 5, 6, 7 mutation occur in the course of precancerous lesion to cancer. Loss of heterozygosity (LOH) of exon4, intron6, APC was 47, 37% (9/19), 8.73% (2/23), 16.67% (3/18) respectively. LOH of exon4 had something to do with poor differentiation, lymph node metastasis, depth of invasion. LOH of exon4 may be of prognostic marker of gastric cancer. We are led to conclude that p53 gene mutation is an early event and perhaps work together with ras oncogene in gastric carcinogenesis.

References

Jan 1, 1992·Journal of Cancer Research and Clinical Oncology·H YokozakiE Tahara
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R F BoyntonS J Meltzer
Jan 1, 1993·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·H TohdoE Tahara
Jul 9, 1993·International Journal of Cancer. Journal International Du Cancer·S UchinoS Hirohashi

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Citations

Mar 6, 2003·Human Mutation·C M Fenoglio-PreiserA Noffsinger
Dec 7, 2007·Journal of Cellular and Molecular Medicine·Jian-Xin Gao
Sep 8, 2004·Cancer Genetics and Cytogenetics·Ana Cristina Gobbo CésarAna Elizabete Silva
Jul 19, 2003·Arquivos De Gastroenterologia·Ana Cristina Gobbo CésarEloiza Helena Tajara

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