PMID: 11605784Oct 19, 2001Paper

Abnormal DNA-binding of transcription factors in minimal change nephrotic syndrome

Pediatric Nephrology : Journal of the International Pediatric Nephrology Association
C CaoR Zhao

Abstract

The activation of transcription factors such as nuclear factor kappaB (NF-kappaB) and activator protein 1 (AP-1) plays an important role in regulating the expression of target genes, including those for cytokines involved in pathogenesis of minimal change nephrotic syndrome (MCNS). The therapeutic effects of glucocorticoids depend on the glucocorticoid receptor (GR) acting on gene transcription and interacting with certain transcription factors. To explore the role of transcription factors in the pathogenesis of MCNS and the therapeutic effects of glucocorticoids, we examined the DNA-binding abilities of NF-kappaB, AP-1, and GR in peripheral blood mononuclear cells (PBMC) from 6 children with MCNS and 6 healthy controls by electrophoretic mobility shift assay (EMSA). NF-kappaB and AP-1 DNA-binding abilities were significantly increased both at baseline and after stimulation by phorbol 12-myristate 13-acetate (TPA) in PBMC from MCNS patients compared with controls, but declined to normal levels after treatment with dexamethasone (DEX). GR DNA-binding abilities were significantly reduced at baseline and after treatment with TPA, but were enhanced markedly by DEX. There were strong correlations between urinary protein and the base...Continue Reading

Citations

Feb 19, 2009·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Sema AkmanAyfer Gur Guven
May 2, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Scott E Wenderfer
Jun 18, 2004·Kidney International·Diego H AvilesArnold H Zea
Oct 28, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Seyhan ErisirSema Akman
Mar 25, 2015·Case Reports in Nephrology·Giles WaltersBudhima Nanayakkara
Jul 31, 2007·International Journal of Toxicology·Alireza RazaviAbbas Mirshafiey
Dec 30, 2008·Journal of Biochemical and Molecular Toxicology·XunHui XuJianQiang He
Aug 5, 2004·The Journal of Biological Chemistry·Jonathan LebowitzJohn P Johnson

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