Abnormal DNA Methylation of Imprinted Loci in Human Preimplantation Embryos

Reproductive Sciences
Xiaoyun ShiYaqin Wu


To evaluate the epigenetic risk linked to assisted reproductive technology (ART) at single-embryo level by analyzing the methylation status of imprinted H19, PEG1, and KvDMR1 in human preimplantation embryos. A total of 254 human day 3 embryos produced by ART procedures were collected. All embryos were normally fertilized but unsuitable for transfer or freezing due to poor quality. Pyrosequencing with confirmatory routine bisulfite sequencing were used to determine the DNA methylation patterns of H19 differentially methylated region (DMR) in 63 embryos, PEG1 DMR in 65 embryos, and KvDMR1 in 67 embryos. Aberrant methylation patterns were found in 8.0% embryos at H19 DMR, 16.9% embryos at PEG1 DMR, and 10.4% embryos at KvDMR1. No methylation errors were found in corresponding sperm samples. We hypothesized that the use of poor-quality embryos may increase the risk of imprinting defects because they might have methylation errors.


Dec 3, 2015·Scientific Reports·Carlee R WhiteMellissa R W Mann
Sep 24, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sebastian CanovasPilar Coy
Jan 27, 2019·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Kevin T Militello, Josephine A Reinhardt
Oct 22, 2019·Human Reproduction Update·Maria ArgyrakiAlexandros Lambropoulos
Apr 24, 2020·Human Reproduction·Min YeRi-Cheng Chian
Sep 26, 2018·BJOG : an International Journal of Obstetrics and Gynaecology·J HuntrissRoyal College of Obstetricians Gynaecologists
Apr 27, 2017·Reproductive Sciences·Yudong LiuShiling Chen
Feb 9, 2019·Clinical Epigenetics·Hiromitsu HattoriTakahiro Arima


Nov 20, 2002·American Journal of Human Genetics·Michael R DeBaunAndrew P Feinberg
Dec 8, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Helena FulkaJosef Fulka
Dec 20, 2005·Human Reproduction·A G SutcliffeEamonn R Maher
Aug 23, 2006·Human Reproduction·Akiko SatoTakahiro Arima
Oct 9, 2007·Human Reproduction·Sarah BowdinWilliam Reardon
Apr 3, 2009·Fertility and Sterility·C Joana MarquesMário Sousa
May 28, 2009·European Journal of Human Genetics : EJHG·Hisato KobayashiTakahiro Arima
Jun 25, 2009·European Journal of Human Genetics : EJHG·Rosanna WeksbergJ Bruce Beckwith
Oct 7, 2009·Human Molecular Genetics·Brenna A Market-VelkerMellissa R W Mann
Jan 21, 2010·Human Reproduction·John C DumoulinJohannes L Evers
Mar 23, 2010·Fertility and Sterility·Shiling ChenChen Luo
Apr 7, 2010·Annales d'endocrinologie·Y Le BoucC Gicquel
Jul 17, 2010·Human Reproduction·Fátima SantosWendy Dean
Mar 15, 2011·Best Practice & Research. Clinical Endocrinology & Metabolism·G BinderKai Kannenberg
Jun 10, 2011·European Journal of Human Genetics : EJHG·Samira Ibala-RomdhaneAnnick Lefèvre
Jan 24, 2012·Human Reproduction Update·A P A van MontfoortP de Boer
Nov 21, 2012·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Xiaoyun ShiYaqin Wu

Related Concepts

Hydrogen sulfite
DNA Methylation [PE]
Protein Methylation
Entire Embryo
DNA Methylation
KCNQ1OT1 gene
Study of Epigenetics

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.