Abnormal expansion of peripheral gamma delta T cells in patients with neurologic disorders

Brain, Behavior, and Immunity
T YamamotoK Takeshita

Abstract

The proportions and the variable region usages of peripheral gamma delta T cells were investigated in 205 patients with various neurologic disorders. Flow cytometric analysis was performed with monoclonal antibodies against C delta, V delta 1, V delta 2. V delta 1-J delta 1/J delta 2, and V gamma II (9) epitopes. Further analysis was carried out with reverse transcription-polymerase chain reaction (RT-PCR) using variable region-specific primers. The proportion of gamma delta T cells in 33 patients with cerebral palsy (CP) (mean +/- SD; 11.1 +/- 11.0%) was significantly higher than that in 35 normal controls (5.6 +/- 2.6%) (p < .01). Of 205 patients, persistent gamma delta T cell expansion over 15% was observed in 15 patients (M/F = 13/2). Nine of these 15 were patients with CP. Six of the 9 with CP had neither perinatal nor postnatal events related to the neurologic abnormalities. None of the 9 with CP had active infections or autoimmune disorders that could induce gamma delta T cell expansion. Of the 15, 13 had V gamma II/V delta 2-dominant expansion and 2 showed V delta 1-dominant expansion. Unusual expansions of V delta 4 and V delta 6, which were not usually found in peripheral blood, were detected by RT-PCR in one case wit...Continue Reading

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