PMID: 6987524Feb 28, 1980Paper

Abnormal expression of chromosomal rabbit beta-globin gene in Saccharomyces cerevisiae

Nature
J D BeggsC Weissmann

Abstract

A 5.1 kilobase-pair segment of rabbit chromosomal beta-globin DNA was joined to pJDB219, a plasmid consisting of pMB9, the 2-mu yeast plasmid and the yeast leu-2 gene. Saccharomyces cerevisiae transformed with the globin DNA-containing hybrid produced beta-globin-specific RNA. As compared to mature beta-globin mRNA, these transcripts lacked 20-40 nucleotides from the 5' end, contained all of the small intron and extended to about the middle of the large intron. Thus, no splicing of the primary beta-globin transcript could be detected in the yeast cells.

References

Dec 1, 1976·Analytical Biochemistry·T C Currier, E W Nester
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Jan 1, 1979·Annual Review of Biochemistry·J Abelson
Oct 25, 1979·Nucleic Acids Research·N M WilkieC Weissmann
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K StruhlR W Davis
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J C AlwineG R Stark
Jan 1, 1975·Methods in Cell Biology·D R CryerJ Marmur
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Grunstein, D S Hogness
Nov 1, 1973·Journal of Bacteriology·P GuerryS Falkow

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Citations

Aug 1, 1990·Molecular & General Genetics : MGG·T AshikariK Yano
Jan 1, 1981·Molecular & General Genetics : MGG·J B McNeil, J D Friesen
Sep 1, 1984·Gene·I R BeachamJ Carbon
Dec 2, 1996·Molecular and Biochemical Parasitology·S Metzenberg, N Agabian
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W F AndersonE G Diacumakos
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S KitM Hazen
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·V A Zakian
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G L McKnight, B L McConaughy
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C LangfordD Gallwitz
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T L Smith
Jan 1, 1985·Biotechnology & Genetic Engineering Reviews·S M KingsmanN A Roberts
Mar 25, 1983·Nucleic Acids Research·R DerynckD V Goeddel
Nov 11, 1985·Nucleic Acids Research·L P WoudtR J Planta
Mar 1, 1984·European Journal of Biochemistry·R Hörcher, K H Seifart
Apr 15, 1986·European Journal of Biochemistry·G J BelshamA E Smith
Aug 28, 1980·Nature·J L Hartley, J E Donelson
Oct 29, 1981·Nature·R A HitzemanB D Hall
Jun 1, 1992·Yeast·M A RomanosJ J Clare
Sep 1, 1984·Bioscience Reports·R W Davies
Oct 26, 1999·Infection and Immunity·C F ThomasA H Limper
May 1, 1989·Molecular and Cellular Biology·J O DeshlerJ J Rossi
Dec 1, 1988·Molecular and Cellular Biology·P BrennwaldJ A Wise
Nov 1, 1984·Molecular and Cellular Biology·R L LastJ L Woolford

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