Abnormal expression of key genes and proteins in the canonical Wnt/β-catenin pathway of articular cartilage in a rat model of exercise-induced osteoarthritis

Molecular Medicine Reports
Shen-Shen LiuYanqiu Zhang

Abstract

To investigate the molecular pathogenesis of the canonical Wnt/β-catenin pathway in exercise-induced osteoarthritis (OA), 30 male healthy Sprague Dawley rats were divided into three groups (control, normal exercise‑induced OA and injured exercise‑induced OA groups) in order to establish the exercise‑induced OA rat model. The mRNA and protein expression levels of Runx‑2, BMP‑2, Ctnnb1, Sox‑9, collagen Ⅱ, Mmp‑13, Wnt‑3a and β‑catenin in chondrocytes were detected by reverse transcription‑quantitative polymerase chain reaction, western blotting and immunohistochemical staining. The mRNA levels of Runx‑2, BMP‑2 and Ctnnb1 were upregulated in the normal exercise‑induced OA and injured exercise‑induced OA groups; while Runx‑2 and BMP‑2 were upregulated in the injured exercise‑induced OA group when compared with the normal exercise‑induced OA group. The protein levels of Mmp‑13, Wnt‑3a and β‑catenin were increased and collagen Ⅱ was reduced in the normal exercise‑induced OA and injured exercise‑induced OA groups. Ctnnb1, Wnt‑3a and β‑catenin, which are key genes and proteins in the canonical Wnt/β‑catenin pathway, were abnormally expressed in chondrocytes of the exercise‑induced OA rat model. Ctnnb1, β‑catenin and Wnt‑3a were suggeste...Continue Reading

References

Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Wodarz, R Nusse
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 6, 2004·Science·W James Nelson, Roel Nusse
Aug 18, 2004·Rejuvenation Research·T AignerJ Buckwalter
Aug 18, 2004·Current Opinion in Rheumatology·Joseph A Buckwalter, James A Martin
Feb 3, 2005·Cell Research·Roel Nusse
Feb 26, 2005·Annals of the Rheumatic Diseases·M J BenitoB Bresnihan
Aug 16, 2005·FEBS Letters·Sang-Gu HwangJang-Soo Chun
Sep 9, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Peter S BurrageConstance E Brinckerhoff
Apr 28, 2006·Journal of Anatomy·F EcksteinR Putz
Aug 9, 2006·Arthritis Research & Therapy·Francesco Dell'AccioCostantino Pitzalis
Jan 30, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takahito YuasaMotomi Enomoto-Iwamoto
Mar 28, 2008·Archives of Biochemistry and Biophysics·Elke PitersWim Van Hul
Sep 5, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mei ZhuDi Chen
Nov 20, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hiroshi Kawaguchi
Dec 3, 2008·The Journal of Biological Chemistry·Lilia TopolYingzi Yang
Jan 13, 2009·Bone·Frank P LuytenRik J Lories
Oct 7, 2010·Nature Reviews. Rheumatology·Jérémie Sellam, Francis Berenbaum
Mar 7, 2012·Arthritis and Rheumatism·Richard F LoeserMary B Goldring
Jul 19, 2013·Annals of the Rheumatic Diseases·Yolande F M RamosIngrid Meulenbelt

❮ Previous
Next ❯

Citations

Jan 14, 2017·International Journal of Molecular Sciences·Sara CheleschiAntonella Fioravanti
Feb 18, 2017·Current Opinion in Hematology·Lina Badimon, Maria Borrell-Pages
Aug 20, 2019·Cell Communication and Signaling : CCS·Yudan WangFaming Tian
May 28, 2020·International Journal of Molecular Sciences·Sara CheleschiAntonella Fioravanti
Mar 25, 2019·Journal of Cellular and Molecular Medicine·Selene Pérez-GarcíaRosa P Gomariz
Nov 26, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Aoran HuangLi Yao
Dec 15, 2020·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jin Mi ChunJoong-Sun Kim
Jun 3, 2021·International Journal of Molecular Sciences·Alessandro de SireAntonio Ammendolia
Jul 13, 2021·Frontiers in Cell and Developmental Biology·Pranidhi BaddamDaniel Graf

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Protein Extraction
electrophoresis
X-ray

Software Mentioned

Rad iQ5 software
- Pro Analyzer
Gel
SPSS
Bio

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.