Abnormal expression of the Notch and Wnt/β-catenin signaling pathways in stem-like ALDH(hi)CD44(+) cells correlates highly with Ki-67 expression in breast cancer

Oncology Letters
Junwei CuiWei Wei

Abstract

Previous studies have reported that breast cancer stem cells may be closely associated with tumor metastasis, recurrence, and even the failure of chemotherapy and radiotherapy. The aim of the present study was to investigate whether important cell signaling pathways associated with drug resistance are activated in stem-like acetaldehyde dehydrogenase (ALDH)(hi) cluster of differentiation (CD)44(+) cells, and to analyze the association between ALDH(hi)CD44(+) cells and specific pathological features. ALDH(hi)CD44(+) cells and non-stem-like ALDH(low)CD44(+) cells were separated from MDA-MB-231 cells by fluorescence-activated cell sorting, and the mRNA expression levels of Notch1 and β-catenin were estimated by performing quantitative polymerase chain reaction in the stem-like and non-stem-like cells. Line correlation analysis was used to evaluate the correlation between an immunohistochemical panel of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and Ki-67, and ALDH(hi)CD44(+) cells from patients with invasive breast carcinoma. The mRNA levels of Notch1 and β-catenin were significantly higher in the ALDH(hi)CD44(+) cells compared with those in the ALDH(low)CD44(+) cells (P<0.0...Continue Reading

References

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Citations

Jul 5, 2019·The Kaohsiung Journal of Medical Sciences·Er-Dong Shen, Qiang Zeng
Oct 9, 2015·Clinical & Experimental Metastasis·Mauricio Rodriguez-Torres, Alison L Allan
May 20, 2020·Frontiers in Oncology·Saghar YousefniaKamran Ghaedi
Aug 28, 2020·Frontiers in Immunology·Lia WalcherUta Kossatz-Boehlert
Dec 1, 2018·Pathology Oncology Research : POR·Maria João CarvalhoCarlos Oliveira
Jan 10, 2021·Pharmacology & Therapeutics·Juntao LiErxi Wu

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Methods Mentioned

BETA
fluorescence-activated cell sorting
flow cytometry
PCR

Software Mentioned

SPSS

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