Abnormal inhibition of osteoclastogenesis by mesenchymal stem cells through the miR-4284/CXCL5 axis in ankylosing spondylitis

Cell Death & Disease
Wenjie LiuHuiyong Shen

Abstract

Ankylosing spondylitis (AS) is a common inflammatory autoimmune disease, characterized by pathological osteogenesis. Mesenchymal stem cells (MSCs), as the main source of osteoblasts, participate in bone remodeling not only through differentiation into osteoblasts but also through indirect regulation of osteoclastogenesis. Our previous study indicated that the stronger osteogenic differentiation of MSCs from AS patients (ASMSCs) involved in pathological osteogenesis. However, whether there is any abnormality in the regulation of osteoclastogenesis by ASMSCs remains unclear. In this study, ASMSCs or MSCs from healthy donors (HDMSCs) were co-cultured with CD14 + monocytes in osteoclast induction medium. Our results demonstrated that ASMSCs exhibited a stronger capacity to inhibit osteoclastogenesis than HDMSCs. To explore underlying mechanisms, cytokine array assays were performed, showing that ASMSCs secreted more CXCL5 than HDMSCs, which was confirmed by enzyme-linked immunosorbent assays. Moreover, inhibition of osteoclastogenesis by ASMSCs was recovered by decreasing CXCL5. Besides, the inhibitory effect of CXCL5 on osteoclastogenesis was confirmed by exogenous addition. Bioinformatics analysis was applied to find the interact...Continue Reading

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Citations

Jul 28, 2020·Journal of Cellular and Molecular Medicine·Wenjie LiuYanfeng Wu
Feb 10, 2021·Journal of Cellular Physiology·Lifei LiuJun Zou
Feb 12, 2021·Seminars in Immunopathology·Daniele MauroFrancesco Ciccia
Apr 7, 2021·Connective Tissue Research·Brett S KlosterhoffNick J Willett

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Methods Mentioned

BETA
density-gradient centrifugation
Profiler
ELISA
Assay
flow cytometry
transfection

Software Mentioned

HLImage
SPSS

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