Abnormal levels of transcript abundance of developmentally important genes in various stages of preimplantation bovine somatic cell nuclear transfer embryos

Cellular Reprogramming
K I AstonK L White

Abstract

Based on microarray data comparing gene expression of fibroblast donor cells and bovine somatic cell nuclear transfer (SCNT) and in vivo produced (AI) blastocysts, a group of genes including several transcription factors was selected for evaluation of transcript abundance. Using SYBR green-based real-time polymerase chain reaction (Q-PCR) the levels of POU domain class 5 transcription factor (Oct4), snail homolog 2 (Snai2), annexin A1 (Anxa1), thrombospondin (Thbs), tumor-associated calcium signal transducer 1 (Tacstd1), and transcription factor AP2 gamma (Tfap2c) were evaluated in bovine fibroblasts, oocytes, embryos 30 min postfusion (SCNT), 12 h postfertilization/activation, as well as two-cell, four-cell, eight-cell, morula, and blastocyst-stage in vitro fertilized (IVF) and SCNT embryos. For every gene except Oct4, levels of transcript were indistinguishable between IVF and SCNT embryos at the blastocyst stage; however, in many cases levels of these genes during stages prior to blastocyst differed significantly. Altered levels of gene transcripts early in development likely have developmental consequences downstream. These results indicate that experiments evaluating gene expression differences between control and SCNT bla...Continue Reading

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Citations

Jun 5, 2013·Zygote : the Biology of Gametes and Early Embryos·Li-Bing MaTeng Cheng
Oct 12, 2010·Molecular Reproduction and Development·Kristin M Whitworth, Randall S Prather
Oct 30, 2013·Molecular Reproduction and Development·Charles R LongMichael C Golding
Sep 23, 2014·Molecular Reproduction and Development·Priscila Di Paula Bessa SantanaMoysés dos Santos Miranda
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Feb 20, 2016·PloS One·Carla Paola BuemoDaniel Felipe Salamone
Apr 7, 2017·Molecular Reproduction and Development·Guan-Nan WangShi-Jie Li
Jan 23, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Yun FengFenghua Lu

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Methods Mentioned

BETA
biopsy
chip
PCR
chips

Software Mentioned

limma
Primer3

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