Abnormal Mechanical Loading Induces Cartilage Degeneration by Accelerating Meniscus Hypertrophy and Mineralization After ACL Injuries In Vivo

The American Journal of Sports Medicine
Guoqing DuL Wei

Abstract

Although patients with an anterior cruciate ligament (ACL) injury have a high risk of developing posttraumatic osteoarthritis (PTOA), the role of meniscus hypertrophy and mineralization in PTOA after an ACL injury remains unknown. The purpose of this study was to determine if menisci respond to abnormal loading and if an ACL injury results in meniscus hypertrophy and calcification. The hypotheses were that (1) abnormal mechanical loading after an ACL injury induces meniscus hypertrophy and mineralization, which correlates to articular cartilage damage in vivo, and (2) abnormal mechanical loading on bovine meniscus explants induces the overexpression of hypertrophic and mineralization markers in vitro. Controlled laboratory study. In vivo guinea pig study (hypothesis 1): Three-month-old male Hartley guinea pigs (n = 9) underwent ACL transection (ACLT) on the right knee; the left knee served as the control. Calcification in the menisci was evaluated by calcein labeling 1 and 5 days before knee harvesting at 5.5 months. Cartilage and meniscus damage and mineralization were quantified by the Osteoarthritis Research Society International score and meniscus grade, respectively. Indian hedgehog (Ihh), matrix metalloproteinase-13 (MMP-...Continue Reading

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Citations

Apr 2, 2016·Current Rheumatology Reports·Elizabeth Mitton-FitzgeraldAnn K Rosenthal
Apr 13, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Mieke NickienCorrinus C van Donkelaar
May 18, 2017·BMC Musculoskeletal Disorders·P BehrendtB Kurz
Dec 10, 2020·Scientific Reports·Yehan FangJianping Lin
Mar 6, 2021·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Peter AngeleJohannes Zellner

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Methods Mentioned

BETA
fluorescence microscopy
enzyme-linked
Assay
biopsy
PCR
Fluorescence
Protein Assay
electrophoresis
ELISA

Software Mentioned

Primers Express
Image
Image Acquisition and Analysis Software ( UVP
BioTools
Pro Plus
Elements AR
MJ
SPSS
HIS

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