Abnormal membrane protein methylation and merocyanine 540 fluorescence in sickle erythrocyte membranes

Biochemical Medicine
C MannaS Kim

Abstract

Sickle cell erythrocytes exhibit reduced carboxyl methylation of membrane proteins compared to normal erythrocytes. This altered methylation in sickle membrane proteins is also observable when extracted membranes, both intact and alkali treated, were used as substrates for the homologous protein methylase II (S-adenosylmethionine:protein-carboxyl O-methyltransferase, EC. 2.1.1.24). However, when glycophorin A, one of the major methyl acceptors in both membranes, was extracted by lithium diiodosalicylate and used as the methyl acceptor, the proteins from both membranes were methylated equally, suggesting an involvement of membrane structure in membrane-bound protein methylation. Merocyanine 540 (MC-540), a fluorescent probe, was used to determine if the membranes differed in organization. Incubation of both normal and sickle erythrocytes membranes with MC-540 produced a marked increase in extrinsic fluorescence, reflecting a relatively nonpolar environment for the dye bound to the membranes. The fluorescence from sickle cell ghosts was only 87% as intense as that from normal ghosts, while the actual amount of MC-540 associated with sickle cell membranes was only 62% of normal. These data suggest that differences exist in the dis...Continue Reading

References

Feb 14, 1975·Biochimica Et Biophysica Acta·S FischerI Tellez-Nagel
Jan 1, 1977·British Journal of Haematology·J Palek
Oct 5, 1978·The New England Journal of Medicine·J Dean, A N Schechter
Mar 1, 1979·The American Journal of Cardiology·A P SelwynG Forse
Jun 1, 1979·European Journal of Biochemistry·P GallettiS Kim
Jan 1, 1978·Journal of Supramolecular Structure·L S LessinH Weems
Apr 1, 1977·The Journal of Clinical Investigation·T AsakuraE Schwartz
Jan 1, 1973·Journal of Supramolecular Structure·T L Steck, J Yu
Aug 1, 1983·Journal of Biochemical and Biophysical Methods·S KimG J Jun
Dec 1, 1983·Biochemical Medicine·J Y RoS Kim
Jun 1, 1981·The Journal of Clinical Investigation·B LubinL L Van Deenen
Nov 25, 1949·Science·L PAULING, H A ITANO

Citations

Dec 1, 1987·Photochemistry and Photobiology·F Sieber
Dec 16, 2006·Retrovirology·Nicole M WillemsenDavid Harrich

Related Concepts

Merocyanine dye
Anemia, Sickle Cell
Serum Proteins
Red Cell Ghost
Abnormal Red Blood Cell
Fluorochromes
Glycophorin E
Cell Surface Proteins
Methylation
Pyrimidones

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