Abnormal membrane surface properties during maturation of rat reticulocytes elicited by bleeding as measured by partition in two-polymer aqueous phases

British Journal of Haematology
H WalterG S Ascher

Abstract

Partition of cells in two-polymer aqueous phases is an extremely sensitive method for the separation of cells and for tracing subtle changes in the cells' membrane surface properties (primarily surface charge) as a function of in vivo processes (e.g. differentiation, maturation, ageing). Combining isotope labelling and cell countercurrent distribution techniques we have examined the membrane surface properties of rat reticulocytes produced in response to erythroid stress by bleeding. It was found that the rapid increase in the partition coefficient of normal reticulocytes subsequent to release into the peripheral blood (which reflects a rapid increase in membrane surface charge) is absent in reticulocytes produced in response to bleeding. In this way the reticulocytes behave like those produced in response to repeated phenylhydrazine injections. 'Stress reticulocytes' never mature to erythrocytes having normal membrane surface properties as judged by partition. The experiments show that our previous results are not due to the phenylhydrazine per se but rather to the production of 'stress reticulocytes'. Further, if remodelling of the phlebotomy-induced reticulocyte cell membrane occurs in the circulation as has been suggested i...Continue Reading

References

Dec 3, 1971·Nature·D E Brooks, T Mann
Dec 1, 1974·British Journal of Haematology·O Gasko, D Danon
Apr 5, 1972·Nature: New Biology·S E ComeS H Robinson
Feb 28, 1973·Biochimica Et Biophysica Acta·R ReithermanS H Barondes
May 1, 1966·Biological Reviews of the Cambridge Philosophical Society·H Borsook
Jan 1, 1969·British Journal of Haematology·A GanzoniC A Finch
Jan 4, 1966·Biochimica Et Biophysica Acta·H Walter, F W Selby
Feb 7, 1967·Biochimica Et Biophysica Acta·H WalterR Garza
Aug 16, 1962·The New England Journal of Medicine·F STOHLMAN
Nov 1, 1962·Experimental Cell Research·P A ALBERTSSON, G D BAIRD

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