Abnormal p53 expression is rare in clinically localized human prostate cancer: comparison between immunohistochemical and molecular detection of p53 mutations

The Prostate
A E MottazU E Studer

Abstract

We assessed the frequency and molecular basis of p53 mutations in clinically localized prostatic adenocarcinoma. Prostate specimens were examined from 100 patients with clinically localized prostatic adenocarcinoma and 13 patients with benign prostatic hyperplasia (BPH). Mutations producing nuclear accumulation of p53 were detected immunohistochemically. Exon-specific mutations were analyzed by polymerase chain reaction amplification and single strand conformation polymorphism (PCR-SSCP) and sequenced. p53 accumulation was detected in 5 tumors using antibody DO-1, and in 4 of these using antibody PAb 1801, but not in BPH. PCR-SSCP detected mutations in all 5 tumors, with alterations in exon 5 for 1 tumor, exon 6 for 3 tumors, and exon 7 for 1 tumor. An exon 6 mutation was also found in a tumor with no anti-p53 staining. p53 mutations are uncommon in clinically localized prostatic adenocarcinoma and absent from BPH. 5 of the 6 mutations were derived from locally invasive, prostate carcinomas, supporting the hypothesis that mutation of p53 is a late event in prostate carcinoma progression.

References

Jun 3, 1992·Journal of the National Cancer Institute·A D ThorB H Mayall
Jun 3, 1992·Journal of the National Cancer Institute·T VisakorpiJ Isola
Jul 1, 1991·Analytical Biochemistry·B J BassamP M Gresshoff
Jun 6, 1991·Nature·A J LevineC A Finlay
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M OritaT Sekiya
Jun 15, 1994·Journal of the National Cancer Institute·S G ChiP H Gumerlock
Mar 1, 1994·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·F LeviC La Vecchia
Jul 1, 1995·The Prostate·Y KubotaK Danenberg
Feb 1, 1994·The Journal of Urology·H J VoellerE P Gelmann
Jan 1, 1993·The Prostate·A G CarrollE P Gelmann
Nov 1, 1993·British Journal of Urology·D J ThomasD E Neal
Jan 1, 1993·The Prostate·P J Van VeldhuizenR L Stephens

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Citations

Jan 12, 1999·British Journal of Urology·W D DunsmuirR S Kirby
Jan 20, 2007·BJU International·Abdel-Rahmene AzzouziFreddie C Hamdy
Feb 28, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Wanzhong WangJan-Erik Damber
Oct 21, 2004·Cancer·David G BostwickBarry Timms
May 16, 2001·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·K R LeiteL H Camara-Lopes
Jan 6, 2001·Scandinavian Journal of Urology and Nephrology. Supplementum·C S FosterW A Sakr
Mar 5, 2004·Prostate Cancer and Prostatic Diseases·M K Karayi, A F Markham
May 8, 1999·BJU International·C S FosterY Ke
Oct 4, 2014·Oncotarget·Emma ShtivelmanChristopher P Evans
Oct 5, 2001·Disease-a-month : DM·J A Eastham, P T Scardino

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