PMID: 11607118Nov 1, 1990Paper

Abnormal plant development and down-regulation of phenylpropanoid biosynthesis in transgenic tobacco containing a heterologous phenylalanine ammonia-lyase gene

Proceedings of the National Academy of Sciences of the United States of America
Y ElkindC Lamb

Abstract

Biosynthesis of phenylpropanoid natural products in tobacco was perturbed by introduction of a heterologous (bean) phenylalanine ammonia-lyase (PAL; L-phenylalanine ammonia-lyase, EC 4.3.1.5) gene, modified by inclusion of cauliflower mosaic virus 35S enhancer sequences in its promoter. These transgenic plants can exhibit a series of unusual phenotypes including localized fluorescent lesions, altered leaf shape and texture, reduced signification in xylem, stunted growth, reduced pollen viability, and altered flower morphology and pigmentation. Genetic analysis of a transformant with severe symptoms showed that symptom development was inherited as a single, partially dominant trait and cosegregated with reduced levels of PAL activity and soluble phenylpropanoid products. Accumulation of transcripts encoded by the endogenous tobacco PAL genes was suppressed. We conclude that the transgene disrupts PAL regulation and that some of the phenotypes reflect interference with putative signals dependent on phenylpropanoid biosynthesis.

References

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Citations

Sep 1, 1993·Plant Molecular Biology·F F AssaadE R Signer
Apr 9, 2005·Plant Cell Reports·Keiko YoshinagaMaki Kawai-Yamada
Apr 6, 2012·Plant Cell Reports·Yuepeng SongDeqiang Zhang
Jun 30, 2001·Phytochemistry·A Kawaoka, H Ebinuma
Jun 18, 2003·Phytochemistry·Nicholas J WaltonArjan Narbad
Mar 6, 1999·Current Opinion in Plant Biology·C Chapple, N Carpita
Jun 8, 2001·The Plant Journal : for Cell and Molecular Biology·L JonesS R Turner
Aug 17, 2002·Molecular Plant-microbe Interactions : MPMI·Ezequiel Balmori-MelianRichard L S Forster
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·P. Meyer, H. Saedler
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Ross W. WhettenRonald R. Sederoff
Sep 25, 2003·Annual Review of Plant Biology·Wout BoerjanMarie Baucher
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·H J Jung, W Ni
Aug 16, 2011·Annual Review of Phytopathology·Richard A Dixon
Sep 3, 2010·Annual Review of Genetics·Nicholas D Bonawitz, Clint Chapple
Jan 1, 1994·Antonie van Leeuwenhoek·C CogoniG Macino
Apr 29, 2015·Plant Biotechnology Journal·Cécile M Parmentier-Line, Gary D Coleman
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Xu LiClint Chapple

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