Abnormal sialic acid content of the dysfibrinogenemia associated with liver disease

The Journal of Clinical Investigation
J MartinezD Kwasniak

Abstract

To evaluate the possibility that the carbohydrate composition of fibrinogen may be altered in the dysfibrinogenemia associated with liver disease, we studied the sialic acid content of purified fibrinogen from 12 patients with liver disease and its relationship to the prolongation of the thrombin time. Purified fibrinogen showed that Aalpha-, Bbeta-, and gamma-chains when reduced and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and exhibited prolongation of the thrombin time similar to that of the plasma from which it was derived. Sialic acid content of the purified fibrinogen ranged from 12.7 to 71.4% higher in patient fibrinogens when compared to normal controls. A progressive delay in thrombin time was associated with increasing sialic acid content of the patient fibrinogen. Enzymatic removal of sialic acid from four of the abnormal fibrinogens resulted in a shortening of their thrombin times to the range of the desialylated normal control. Periodic acid-Schiff reagent stained only the Bbeta- and gamma-chains of the reduced patient fibrinogens after sodium dodecyl sulfate-polyacrylamide gel electrophoresis suggesting that the excess sialic acid is located on these two chains. These studies demonstrat...Continue Reading

References

Nov 1, 1976·British Journal of Haematology·G GreenL Poller
Jul 1, 1977·The Journal of Clinical Investigation·J E Palascak, J Martinez
Sep 1, 1974·The Journal of Clinical Investigation·J S MarshallD M Carlson
Jul 1, 1969·Analytical Biochemistry·R M ZachariusJ J Woodlock
Aug 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·L A KAZALL M TOCANTINS

Citations

Nov 2, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·C K LiuS Waxman
May 1, 1984·Thrombosis Research·J L FrancisV J Simmonds
Aug 11, 2000·Journal of Biotechnology·W ChotigeatA Phongdara
Jul 20, 2002·Journal of Hepatology·Ton LismanPhilip G de Groot
Feb 7, 2001·Gastroenterology Clinics of North America·G S MaltzJ L Carson
Jan 1, 1986·Critical Reviews in Oncology/hematology·A Z Budzynski
Aug 3, 1978·The New England Journal of Medicine·H R GralnickE Abrams
Mar 1, 1986·Gut·D A Kelly, E G Tuddenham
Apr 1, 1982·The Journal of Clinical Investigation·G HoldsworthD J Galton
Apr 18, 2015·Indian Journal of Clinical Biochemistry : IJCB·Mohamed HessienBatoul Izz ulArab
Feb 3, 2016·Journal of Thrombosis and Haemostasis : JTH·G C G HugenholtzR A S Ariëns
Apr 1, 1985·The American Journal of Medicine·N A DawsonB M Alving
Oct 28, 2016·Thrombosis and Haemostasis·Ton Lisman, Francesco Violi
Sep 30, 2016·World Journal of Transplantation·Lesley De PietriBruno Begliomini
Oct 13, 2006·International Anesthesiology Clinics·Yoogoo Kang, Paul Audu
Jun 27, 1983·Annals of the New York Academy of Sciences·M W Mosesson
Jun 27, 1983·Annals of the New York Academy of Sciences·J MartinezJ E Palascak
Jan 1, 1982·Alcoholism, Clinical and Experimental Research·M V RagniU Hasiba
Jan 1, 1981·Critical Reviews in Clinical Laboratory Sciences·D A Triplett
Jun 13, 2000·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·S I Rapaport
Aug 1, 1990·American Journal of Hematology·H TakahashiA Shibata
Jan 1, 1987·Annals of the New York Academy of Sciences·L A Harker
Jul 27, 2018·Research and Practice in Thrombosis and Haemostasis·Ton Lisman, Robert J Porte
Jan 10, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Judith J de VriesMoniek P M de Maat
Mar 1, 1979·Klinische Wochenschrift·E KöttgenW Gerok
Mar 1, 1996·The Journal of Biological Chemistry·J L WoodheadJ W Weisel

Related Concepts

Blood Coagulation Disorders
Gamma-Fibrinogen
Liver Dysfunction
Sialic Acids

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