PMID: 8951934Oct 1, 1996Paper

Abnormal spontaneous activity and responses to norepinephrine in dissociated dorsal root ganglion cells after chronic nerve constriction

Pain
M PetersenR H LaMotte

Abstract

As a part of ongoing studies of the cellular mechanisms of sympathetically maintained pain, we investigated the effects of a peripheral nerve injury on the responses of dissociated dorsal root ganglion (DRG) cells to norepinephrine (NE). Whole-cell patch-clamp recordings under current clamp were obtained from L4 and L5 DRG cells from adult rats in acute culture 11-25 days after a loose or tight ligation of the sciatic nerve. Only small to medium-sized cells from normal (uninjured) nerves and from loosely ligated nerves were tested with NE. One of 15 cells obtained-from uninjured nerves responded to NE (500 microM, the highest dose, elicited a small depolarization without action potentials). In contrast, many cells from injured nerves responded to NE with a membrane depolarization, accompanied in some cases by the generation of action potentials. Fifty-two percent responded to 500 microM, while a significantly lower percentage responded to the lower doses of 100 microM (26%) and 10 microM (14%). Cells responsive to NE also responded to capsaicin. Spontaneous activity was observed in 14% and 21% of cells from loosely and tightly ligated nerves, respectively, but none of the cells from uninjured nerves. We conclude that the abnorm...Continue Reading

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Citations

Apr 8, 1998·Journal of Neuroscience Methods·J M ZhangR H LaMotte
Dec 10, 1999·Journal of the Autonomic Nervous System·F A Abdulla, P A Smith
Aug 26, 2003·Advanced Drug Delivery Reviews·Lili X Wang, Zaijie Jim Wang
Feb 13, 1999·Progress in Neurobiology·M J Millan
Jul 1, 2006·Anesthesiology·J Bruce McCallumQuinn H Hogan
Oct 7, 2006·Current Opinion in Gastroenterology·S Srinivasan, J W Wiley
Jan 19, 2002·Regional Anesthesia and Pain Medicine·Constantine SarantopoulosQuinn Hogan
Jan 25, 2008·Clinical and Experimental Pharmacology & Physiology·Gael F GibbsJacqueline K Phillips
Aug 5, 2003·Canadian Journal of Physiology and Pharmacology·Fuad A AbdullaPeter A Smith
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Jul 17, 2001·Journal of Neurosurgery·H A Wilkinson, A S Chan
Jan 15, 2014·Autonomic Neuroscience : Basic & Clinical·Tanja SchlerethFrank Birklein
Dec 28, 2002·Anesthesiology·J Bruce McCallumQuinn H Hogan
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Nov 23, 2006·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Andre MullerR Norman Harden
Oct 13, 2006·Progress in Neurobiology·Antti Pertovaara
Apr 25, 2006·The Journal of Pain : Official Journal of the American Pain Society·Ron AmirGary R Strichartz
Nov 17, 2015·European Journal of Pain : EJP·Eleanor S DrummondPeter D Drummond
Dec 25, 2010·Neuroscience·L F DawsonP D Drummond

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