Abnormal thymic stromal lymphopoietin expression in the duodenal mucosa of patients with coeliac disease

Gut
Paolo BiancheriGino R Corazza

Abstract

The short isoform of thymic stromal lymphopoietin (TSLP), a cytokine constitutively expressed by epithelial cells, is crucial in preserving immune tolerance in the gut. TSLP deficiency has been implicated in sustaining intestinal damage in Crohn's disease. We explored mucosal TSLP expression and function in refractory and uncomplicated coeliac disease (CD), a T-cell-mediated enteropathy induced by gluten in genetically susceptible individuals. TSLP isoforms-long and short-and receptors-TSLPR and interleukin (IL)-7Rα-were assessed by immunofluorescence, immunoblotting and qRT-PCR in the duodenum of untreated, treated, potential and refractory patients with CD. The ability of the serine protease furin or CD biopsy supernatants to cleave TSLP was evaluated by immunoblotting. The production of interferon (IFN)-γ and IL-8 by untreated CD biopsies cultured ex vivo with TSLP isoforms was also assessed. Mucosal TSLP, but not TSLPR and IL-7Rα, was reduced in untreated CD and refractory CD in comparison to treated CD, potential CD and controls. Transcripts of both TSLP isoforms were decreased in active CD mucosa. Furin, which was overexpressed in active CD biopsies, was able to cleave TSLP in vitro. Accordingly, refractory and untreated ...Continue Reading

References

Oct 27, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A G UpadhyaS M Strasberg
May 2, 2002·Clinical and Experimental Immunology·R CiccocioppoG R Corazza
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Gary Thomas
Jun 14, 2003·Gastroenterology·Jan-Michel OtteDaniel K Podolsky
Nov 5, 2004·Current Opinion in Pharmacology·Sylvia L F Pender, Thomas T MacDonald
Dec 21, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Rachele CiccocioppoDetlef Schuppan
May 27, 2005·Journal of Clinical Pathology·G R Corazza, V Villanacci
Sep 6, 2005·Nature Immunology·Baohua ZhouSteven F Ziegler
Mar 3, 2007·Pharmacology & Therapeutics·Akira AndohNicholas A Wright
Sep 26, 2007·The Journal of Experimental Medicine·Iliyan D IlievMaria Rescigno
Feb 21, 2009·BMJ : British Medical Journal·Roger Jones, Sarah Sleet
Apr 28, 2009·Lancet·Antonio Di Sabatino, Gino Roberto Corazza
Sep 5, 2009·The Journal of Clinical Investigation·Maria Rescigno, Antonio Di Sabatino
Dec 18, 2009·Mucosal Immunology·M R Comeau, S F Ziegler
Jan 12, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ivan MonteleoneGiovanni Monteleone
Feb 12, 2010·Annals of the New York Academy of Sciences·Rui He, Raif S Geha
Mar 20, 2010·Nature Immunology·Steven F Ziegler, David Artis
Mar 25, 2010·Gut·Alberto Rubio-Tapia, Joseph A Murray
Jun 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mohit KashyapWarren J Leonard
Jan 25, 2012·Allergology International : Official Journal of the Japanese Society of Allergology·Toshiro Takai
Mar 20, 2012·Cell and Tissue Research·Paolo BiancheriThomas T MacDonald
Mar 24, 2012·Journal of Leukocyte Biology·Florence RoanSteven F Ziegler
Feb 16, 2013·Annals of the Rheumatic Diseases·Alicia UsateguiJosé L Pablos
May 22, 2013·The Journal of Allergy and Clinical Immunology·Deepti R NagarkarAtsushi Kato
Oct 2, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arnab DattaRachel C Chambers
Oct 16, 2013·Clinical Science·Roberta CarusoGiovanni Monteleone
May 28, 2015·The Journal of Allergy and Clinical Immunology·Giulia FornasaMaria Rescigno

❮ Previous
Next ❯

Citations

Aug 16, 2015·Autoimmunity Reviews·Antonio Di SabatinoGino Roberto Corazza
May 1, 2016·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Antonio Di SabatinoGino Roberto Corazza
Mar 10, 2017·Cellular and Molecular Gastroenterology and Hepatology·Katerina TsilingiriMaria Rescigno
Apr 4, 2017·Nature Communications·Kenneth VerstraeteSavvas N Savvides
Sep 25, 2019·Expert Opinion on Investigational Drugs·Giancarlo MaroneGilda Varricchi
Jul 13, 2018·Frontiers in Medicine·Antonio Di SabatinoCarmen Gianfrani
Jul 31, 2018·Frontiers in Immunology·Gilda VarricchiGianni Marone
Apr 2, 2019·The Journal of Clinical Investigation·Florence RoanSteven F Ziegler
Aug 28, 2020·Frontiers in Immunology·Iva Marković, Savvas N Savvides
Jan 25, 2020·Allergology International : Official Journal of the Japanese Society of Allergology·Saeko NakajimaKoichiro Asano
Mar 1, 2021·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Shouquan DongQiu Zhao
Aug 8, 2020·Pharmacology & Therapeutics·Partho Protim AdhikarySarah Hedtrich
Aug 20, 2021·Frontiers in Immunology·Gilda VarricchiGiuseppe Spadaro

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
light microscopy
thermal dissociation
immunoprecipitation
biopsy
ELISA

Software Mentioned

GraphPad
GraphPad Prism

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.