Abnormally high expression of POLD1, MCM2, and PLK4 promotes relapse of acute lymphoblastic leukemia

Medicine
Sheng LiGuiqin Zhang

Abstract

This study aimed to explore the underlying mechanism of relapsed acute lymphoblastic leukemia (ALL).Datasets of GSE28460 and GSE18497 were downloaded from Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) between diagnostic and relapsed ALL samples were identified using Limma package in R, and a Venn diagram was drawn. Next, functional enrichment analyses of co-regulated DEGs were performed. Based on the String database, protein-protein interaction network and module analyses were also conducted. Moreover, transcription factors and miRNAs targeting co-regulated DEGs were predicted using the WebGestalt online tool.A total of 71 co-regulated DEGs were identified, including 56 co-upregulated genes and 15 co-downregulated genes. Functional enrichment analyses showed that upregulated DEGs were significantly enriched in the cell cycle, and DNA replication, and repair related pathways. POLD1, MCM2, and PLK4 were hub proteins in both protein-protein interaction network and module, and might be potential targets of E2F. Additionally, POLD1 and MCM2 were found to be regulated by miR-520H via E2F1.High expression of POLD1, MCM2, and PLK4 might play positive roles in the recurrence of ALL, and could serve as potential th...Continue Reading

References

Nov 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert E GoldsbyBradley D Preston
Feb 13, 2004·Bioinformatics·Laurent GautierRafael A Irizarry
Dec 21, 2004·Nucleic Acids Research·G Joshi-TopeL Stein
Jun 28, 2005·Nucleic Acids Research·Bing ZhangJay Snoddy
Jan 15, 2008·Journal of Cellular Biochemistry·Rongxia LiaoShaoxiang Zhang
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
May 14, 2009·Stem Cells and Development·Michaela MerkerovaHana Bruchova
Apr 14, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gesche TallenArend von Stackelberg
May 27, 2010·Cancer Research·Jen-Liang SuMien-Chie Hung
Oct 14, 2010·American Journal of Hematology·Violaine Havelange, Ramiro Garzon
May 4, 2013·Journal of Cell Science·Katharina F SonnenErich A Nigg
Feb 7, 2014·Human Molecular Genetics·Laura ValleIgnacio Blanco
Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Simon M CarrNicholas B La Thangue
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Jan 6, 2015·Leukemia Research·Özlem TüfekçiYusuf Baran
Jan 28, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jun J YangMary V Relling
Mar 22, 2015·Journal of Cell Science·Lukas ČajánekErich A Nigg
May 12, 2015·Nature Genetics·Kajsa PaulssonBertil Johansson
Jul 3, 2015·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Fernando BellidoLaura Valle
Jan 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Marie-Claude SincennesTrang Hoang
Apr 27, 2016·Current Biology : CB·Sihem ZitouniMónica Bettencourt-Dias
Sep 23, 2016·Proceedings of the National Academy of Sciences of the United States of America·Koichi OshimaAdolfo A Ferrando
Nov 23, 2016·Cancer Research·Ling-Wen DingH Phillip Koeffler

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Citations

Sep 8, 2019·Journal of Cancer Research and Clinical Oncology·Yi Zhao, Xin Wang
Apr 12, 2019·Interdisciplinary Sciences, Computational Life Sciences·Anil Kumar TomarBishwajit Kundu
Jan 22, 2020·Cancer Gene Therapy·Liang QuanChaozeng Si

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Datasets Mentioned

BETA
GSE28460
GSE18497

Methods Mentioned

BETA
ubiquitination
SeT
glycosylation

Software Mentioned

R
Limma package
Cytoscape
Gene SeT Analysis Toolkit
DAVID
WebGestalt
VENNY
CytoNCA
Affy package R

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