PMID: 8948131Oct 25, 1996Paper

ABO genotyping by mutagenically separated polymerase chain reaction

Forensic Science International : Synergy
J C LeeJ G Chang

Abstract

ABO blood groups were determined by the mutagenically separated polymerase chain reaction (MS-PCR). The products from two sets of PCR reactions using the same program for the nucleotides at positions 261 and 703 from cDNA at the ABO locus were used to distinguish A, B and O alleles. Two forward mutagenic allele-specific primers of different lengths for the ABO polymorphic site were paired with the same reverse primer in each PCR reaction. The 216 bp fragment of the PCR products for the 261th nucleotide was A or B allele-specific and the 195 bp fragment was O allele-specific. The 126 bp fragment of the PCR products for the 703th nucleotide was B allele-specific and the 106 bp fragment was A or O allele-specific. The ABO genotypes were determined by the intersection of the predicted alleles from these two PCR reactions. The PCR products were obtained using 10 ng of DNA in 50 microL of PCR reaction mixture, and electrophoresed in 4% agarose gel. In this study, 265 ABO-phenotype known samples (A: 31, B: 48, AB: 6 and O: 180) in Chinese were used. The results of ABO genotypes were AA: 1, AO: 30, BB: 2, BO: 46, AB: 6 and OO: 180. These results were confirmed by the PCR-RFLP ABO genotyping method. This technique is a simple, rapid, an...Continue Reading

References

Aug 1, 1992·Human Molecular Genetics·P H Johnson, D A Hopkinson
May 17, 1990·Nature·F YamamotoS Hakomori
Jan 1, 1987·Methods in Enzymology·K B Mullis, F A Faloona

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Citations

Mar 25, 2014·Legal Medicine·Takashi Taki, Kazuhiko Kibayashi
Apr 21, 2012·Forensic Science International. Genetics·Xianhua JiangFaye Guo
Dec 20, 2011·Legal Medicine·Tomonori MuroHaruo Takeshita
Jul 30, 2009·Electrophoresis·James Chun-I LeeLi-Chin Tsai
Dec 17, 2009·Journal of Forensic Sciences·Li RuanQingge Li
Oct 17, 2012·Legal Medicine·Kazuho MaedaKatsuyoshi Kurihara
Mar 15, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mohammad M RahmanOsamu Yamato
Nov 22, 2020·Journal of Thrombosis and Haemostasis : JTH·Kenichi OgiwaraDavid Lillicrap

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