PMID: 8600125Sep 1, 1995Paper

Abolition of the receptor potential response of isolated mammalian outer hair cells by hair-bundle treatment with elastase: a test of the tip-link hypothesis

Hearing Research
S PreyerA W Gummer

Abstract

To test the hypothesis that the tip-links of hair-cell stereocilia are essential for mechanoelectrical transduction, tip-links of isolated outer hair cells (OHCs) of the guinea-pig cochlea were eliminated with a proteolytic enzyme, elastase, and the influence on the receptor potential measured with the whole-cell patch-clamp technique. Within 45 s of immersion of the hair bundle in 20 IU/ml elastase, the receptor potential in response to direct deflection of the hair bundle was irreversibly abolished. The electrical input impedance of the cell remained unchanged, implying that the channels of the basolateral membrane were not affected by elastase. The effect of elastase on the receptor potential was comparable to changes seen after mechanically induced hair-bundle damage. As a further control, a putative transduction-channel blocker, dihydrostreptomycin (68 microM), which does not affect tip-links, was applied to the hair bundle. Although the receptor potential was also blocked by dihydrostreptomycin, the effect was reversible. The results suggest that tip-links are required for mechanoelectrical transduction of mammalian OHCs.

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Citations

Jul 26, 2008·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Glen M WatsonPatricia Mire
Jul 17, 2010·Journal of the Association for Research in Otolaryngology : JARO·Pavel Mistrík, Jonathan F Ashmore
May 26, 2004·Hearing Research·H P ZennerA Eiber
Jul 29, 2000·Hearing Research·G M Watson, S Venable-Thibodeaux
Feb 1, 1997·Hearing Research·R K Duncan, J W Grant
Feb 24, 1998·Hearing Research·G M WatsonR R Hudson
Oct 8, 1999·Hearing Research·G M WatsonJ J Repass
Feb 24, 2001·Journal of Anatomy·R K DuncanJ C Saunders
Apr 7, 2005·Hearing Research·Jens MeyerAnthony W Gummer
Jun 10, 2011·Communicative & Integrative Biology·Nozomu MatsumotoFederico Kalinec
Mar 31, 2009·Nature Neuroscience·Maryline BeurgAnthony J Ricci
Feb 1, 1996·Die Naturwissenschaften·M Kössl, M Vater
Aug 12, 2014·Current Opinion in Otolaryngology & Head and Neck Surgery·Kevin W Christie, Daniel F Eberl

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