Abortive infection with Sindbis virus of a Chinese hamster ovary cell mutant defective in phosphatidylserine and phosphatidylethanolamine biosynthesis.

Biochimica Et Biophysica Acta
O KugeM Nishijima

Abstract

The effects of phosphatidylserine starvation on the infection with Sindbis virus (an enveloped RNA virus) have been investigated in a Chinese hamster ovary (CHO) cell mutant (strain PSA-3) which requires exogenously added phosphatidylserine for cell growth because it lacks the ability to synthesize this phospholipid. When PSA-3 cells were grown in the absence of phosphatidylserine, the cellular contents of phosphatidylserine and also phosphatidylethanolamine produced through decarboxylation of phosphatidylserine decreased. Sindbis virus production in the mutant cells decreased immediately upon phosphatidylserine deprivation as did the contents of phosphatidylserine and phosphatidylethanolamine, whereas the cell growth, viability, and syntheses of protein, DNA and RNA remained normal for approx. 40 h phosphatidylserine starvation. Although PSA-3 cells grown without phosphatidylserine for 24 h were able to bind and internalize Sindbis virus almost normally, viral RNA synthesis was greatly reduced in the cells, suggesting that nucleocapsids of internalized Sindbis virus are not normally released into the cytoplasm. Unlike mammalian cell mutants defective in endosomal acidification, PSA-3 cells grown without phosphatidylserine were...Continue Reading

References

Mar 17, 1977·Biochimica Et Biophysica Acta·D PapahadjopoulosR Lazo
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A HeleniusJ L Strominger
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J D Esko, C R Raetz
Jan 1, 1978·Annual Review of Biophysics and Bioengineering·J Lenard
Dec 20, 1979·Biochimica Et Biophysica Acta·P R Cullis, B de Kruijff
Apr 19, 1979·Biochimica Et Biophysica Acta·P R Cullis, A J Verkleij
Jan 1, 1986·Annual Review of Biochemistry·I MellmanA Helenius
Apr 1, 1983·The Journal of Cell Biology·A R RobbinsJ L Marshall
Oct 1, 1984·The Journal of Cell Biology·A R RobbinsI Mellman
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M MerionW S Sly
Feb 1, 1980·The Journal of Cell Biology·A HeleniusE Fries
Feb 1, 1982·Scientific American·K SimonsA Helenius

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Citations

Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·M R SutherlandE L Pryzdial
Aug 17, 2005·Journal of Virology·David A Coil, A Dusty Miller
Dec 12, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Yuzuru Akamatsu
Jul 25, 2006·FEBS Letters·Markus R Wenk
May 17, 2006·Biochemical and Biophysical Research Communications·Kyoko SaitoOsamu Kuge
Sep 14, 2000·The Journal of Biological Chemistry·A LampioL Kääriäinen
Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melissa K CallahanAndrew J Henderson
Aug 1, 1997·Trends in Cell Biology·M NishjimaK Hanada

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