PMID: 9663656Jul 15, 1998Paper

Abortive recombination in Escherichia coli ruv mutants blocks chromosome partitioning

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
K IshiokaH Shinagawa

Abstract

All the ruvA, ruvB and ruvC mutants of Escherichia coli are sensitive to treatments that damage DNA, and are mildly defective in homologous recombination. It has been reported that the ruv mutants form nonseptate, multinuclear filaments after low doses of UV irradiation, dependent on the sfiA gene product. In vitro, the RuvAB complex promotes the branch migration of Holliday junctions, and RuvC resolves the junctions endonucleolytically. After a low UV dose (5 J/m2), both delta ruvAB and delta ruvC mutant cells became filamentous, with their chromosomes aggregated in the central region. This corresponded to an increase in nonmigrating DNA on pulsed field gel electrophoresis of the XbaI digested chromosome. Upon further incubation, they produced a large number of anucleoid cells of normal size. A recA mutation, but not a recB mutation, suppressed these phenotypes of the ruv mutants. The ruv polA12(Ts) double mutants were inviable at the nonpermissive temperature and mimicked the morphological phenotypes of the UV irradiated ruv mutants. ruvA, B and C mutations block chromosome partitioning in UV irradiated cells because the abortive homologous recombination covalently links chromosomes together. There is a recBCD independent pat...Continue Reading

References

Aug 1, 1992·Molecular Microbiology·J J Lin, A Sancar
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C A ParsonsS C West
Feb 1, 1991·Molecular & General Genetics : MGG·F BensonR G Lloyd
May 1, 1989·Journal of Bacteriology·C B RussellF W Dahlquist
Oct 1, 1989·Molecular & General Genetics : MGG·H IwasakiH Shinagawa
Jan 1, 1989·Journal of General Microbiology·J M PeinadoM C Loureiro-Días
Sep 20, 1985·Journal of Molecular Biology·A F Taylor, G R Smith
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D G EnnisD W Mount
Dec 1, 1972·Bacteriological Reviews·B J Bachmann
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·O HuismanS Gottesman
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S Mizusawa, S Gottesman
Jan 1, 1984·Molecular & General Genetics : MGG·R G LloydC E Shurvinton
May 1, 1983·Cell·J W SzostakF W Stahl
Nov 1, 1984·Journal of Bacteriology·A M Chaudhury, G R Smith
Dec 10, 1981·Nature·I D Hickson, P T Emmerson
May 1, 1995·Molecular Microbiology·A Kuzminov
Jan 1, 1994·Critical Reviews in Microbiology·A J Clark, S J Sandler
Jan 1, 1997·Annual Review of Genetics·S C West
Jul 3, 2013·Molekuliarnaia biologiia·I V BakhlanovaD M Baĭtin

❮ Previous
Next ❯

Citations

Jun 19, 2013·Analytical Biochemistry·Sharik R Khan, Andrei Kuzminov
Dec 18, 2002·The EMBO Journal·Brigitte W Trautinger, Robert G Lloyd
Jan 14, 2009·Genetics·Adam S Wilkins, Robin Holliday
Jan 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Silvia AyoraJuan C Alonso
Mar 4, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tatsuya ShibataHideo Shinagawa
Jun 23, 2009·Molecular Microbiology·Christian J RudolphRobert G Lloyd
Jan 27, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ryosuke FujikaneYoshizumi Ishino
Jan 12, 2011·Molecular Microbiology·Mohan BabuAlexander F Yakunin
Jan 23, 2010·DNA Repair·Christian J RudolphRobert G Lloyd
Dec 9, 2008·Molecular Cell·Lorraine S Symington, William K Holloman
Aug 27, 2013·The Journal of Biological Chemistry·Kenji KeyamuraTakashi Hishida
Nov 19, 2011·The Journal of Biological Chemistry·Jasbeer Singh Khanduja, K Muniyappa
Aug 2, 2001·Journal of Molecular Biology·C D PutnamJ A Tainer
Nov 1, 2012·EcoSal Plus·Bénédicte Michel, David Leach
Dec 10, 1999·Microbiology and Molecular Biology Reviews : MMBR·A Kuzminov
Nov 25, 2000·Molecular Cell·G A Cromie, D R Leach
Sep 8, 2004·Biochemical and Biophysical Research Communications·Kazumi MashimoKazuo Yamamoto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.