Abortive replication of influenza virus A/WSN/33 in HeLa229 cells: defective viral entry and budding processes

Virology
C N GujuluvaD P Nayak

Abstract

Since influenza A virus replication is defective in HeLa229 cells but productive in Madin-Darby canine kidney (MDCK) cells, we have investigated the steps in the infectious cycle of A/WSN/33 virus defective in HeLa229 cells. We find that both the entry and exit processes of the infectious cycle were defective in HeLa229 cells. During entry, viral adsorption was apparently normal in HeLa229 cells but a subsequent step(s) involving one or more processes namely the fusion/uncoating and nuclear transport of viral ribonucleoprotein was inefficient and slow compared to those in MDCK cells. Fewer HeLa229 cells were infected at the same multiplicities of infection, resistance to ammonium chloride developed much more slowly and degradation of the incoming virus proteins was delayed when compared to those in MDCK cells. Subsequent to the entry process, there was no significant difference in either the synthesis of viral proteins or the transport, maturation, and membrane insertion of viral glycoproteins although the glycosylation pattern of hemagglutinin was different and the peak protein synthesis was albeit delayed in HeLa229 cells compared to that in MDCK cells. However, there was a major defect in the budding and release of viral par...Continue Reading

Citations

May 11, 2007·Virology Journal·Kshama KumariGillian M Air
Oct 10, 2013·Viruses·Edward C Hutchinson, Ervin Fodor
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Melike LakadamyaliXiaowei Zhuang
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