Abrogated energy-dependent uptake of cisplatin in a cisplatin-resistant subline of the human ovarian cancer cell line IGROV-1

Cancer Chemotherapy and Pharmacology
J MaJ H Schellens

Abstract

The parental IGROV-1 human ovarian adenocarcinoma cell line was intermittently exposed to increasing concentrations of cisplatin to obtain resistant sublines. A stable resistant subline with a resistance factor of 8.4 had been developed after 9 months and 28 passages, which was denoted IGROV(CDDP). A high correlation coefficient of 0.97 was found between the log cell survival and the DNA-adduct peak level during the process of resistance development. IGROV(CDDP) was strongly cross-resistant to carboplatin and doxorubicin and moderately cross-resistant to etoposide, docetaxel, and topotecan. Only minor resistance against 5-fluorouracil was observed, whereas IGROV(CDDP) was not cross-resistant to methotrexate. Intracellular accumulation of cisplatin was 65% lower in IGROV(CDDP) as compared with parental IGROV-1 at 37 degrees C under normal conditions. Coincubation of cisplatin with the Na+/K+-ATPase inhibitor ouabain resulted in a more pronounced decrease in platinum accumulation in IGROV-1 (44% decrease) than in IGROV(CDDP) (26% decrease). Under energy-depleting conditions the accumulation of cisplatin in the parental cell line was approximately 60% lower than that observed under normal (energy [i.e., ATP] rich) culture conditio...Continue Reading

Citations

Aug 9, 2003·Biochemical Pharmacology·Sandrine DaubeufAthanase Visvikis
Nov 5, 1998·Molecular Biology of the Cell·A ChausovskyA D Bershadsky
Sep 24, 1999·Journal of the National Cancer Institute·L R DuskaT Hasan
Oct 17, 2007·Annual Review of Pharmacology and Toxicology·Matthew D HallMichael M Gottesman
Jul 1, 2014·PloS One·Charles J d'AdhemarJohn J O'Leary
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·T NakamuraE Canaani
Apr 5, 2014·Journal of Pharmaceutical Sciences·Denise LawlorBritta Stordal
Nov 28, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Verena SchneiderGanna V Kalayda
Jul 16, 2015·NMR in Biomedicine·Victor D Schepkin
Jun 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kelly K FilipskiAlex Sparreboom
Mar 26, 2021·British Journal of Cancer·Omprakash ShriwasRupesh Dash

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