Abrogation of RAB27A expression transiently affects melanoma cell proliferation.

Pigment Cell & Melanoma Research
Dajiang GuoShweta Tikoo

Abstract

The role of the small GTPase RAB27A as an essential melanosome trafficking regulator in melanocytes is well-accepted. A decade ago, RAB27A was identified as a tumor dependency gene that promotes melanoma cell proliferation. RAB27A has since been linked to another propeller of cancer progression: exosome secretion. We have recently demonstrated that RAB27A is overexpressed in a subset of melanomas. High RAB27A gene and protein expression correlate with poor prognosis in melanoma patients. Mechanistic investigations revealed that the generation of pro-invasive exosomes was RAB27A-dependent and, therefore, silencing RAB27A reduced melanoma cell invasion in vitro and in vivo. However, previous studies have implicated RAB27A to be involved in both proliferation and invasion of melanoma cells. Employing four human cell lines, stratified by RAB27A expression, and one RAB27A-high mouse cell line, we demonstrate in this study that the effects of abrogating RAB27A expression on proliferation are only temporary, in contrast to our previously reported persistent effects on tumor invasion and metastasis. Therefore, we assist in the dissection of the short-term effects of RAB27A knockdown on melanoma cell proliferation versus long-term effec...Continue Reading

References

May 1, 2002·The Journal of Biological Chemistry·Molly StromMiguel C Seabra
Dec 7, 2010·Cell·Uri David AkaviaDana Pe'er
Jun 7, 2014·Pigment Cell & Melanoma Research·Nikolas K HaassWolfgang Weninger
Jun 22, 2017·Methods in Molecular Biology·Loredana SpoerriNikolas K Haass
Dec 18, 2018·International Journal of Cancer. Journal International Du Cancer·Dajiang GuoKimberley A Beaumont
Feb 7, 2019·Journal of Cellular Physiology·Jingjing LiJianda Zhou
Jan 25, 2020·The Journal of Investigative Dermatology·Dajiang GuoShweta Tikoo

❮ Previous
Next ❯

Citations

Oct 20, 2020·Frontiers in Cell and Developmental Biology·Cheila BritoMarta Pojo

❮ Previous
Next ❯

Related Concepts