PMID: 11604448Oct 18, 2001Paper

Abscisic acid-specific binding sites in the flesh of developing apple fruit

Journal of Experimental Botany
D P ZhangY Y Shen

Abstract

Abscisic acid (ABA) specific-binding sites localized in the cytosol were identified and characterized in the flesh of developing apple (Malus pumila L. cv. Starkrimon) fruit. ABA binding activity was scarcely detectable in the microsomes but high ABA binding activity in the cytosolic fraction was detected. The ABA-binding sites possessed a protein nature with both active serine residues and thiol-groups of cysteine residues in their functional binding sites. ABA binding was shown to be saturable, reversible and of high affinity. A Scatchard plot provided evidence for two different ABA binding proteins, one with higher affinity (K(d)=2.3 nM) and the other with lower affinity (K(d)=58.8 nM). Phaseic acid, trans-ABA and (-)-ABA had essentially no affinity for the binding proteins, indicating their stereo-specificity to bind physiologically active cis-(+)-ABA. The time-course, pH- and temperature-dependence of the ABA-binding proteins were determined. It is hypothesized that the detected ABA-binding proteins may be putative ABA-receptors that mediate ABA signals during fruit development.

References

Jan 1, 1974·Methods in Enzymology·T K Hodges, R T Leonard
Dec 1, 1994·Plant Molecular Biology·J GiraudatN Vartanian
Jul 1, 1997·Plant Physiology·S Merlot, J Giraudat
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Jeffrey Leung, Jerome Giraudat
Apr 1, 1973·Plant Physiology·R T LeonardT K Hodges

❮ Previous
Next ❯

Citations

Jan 1, 2002·The Arabidopsis Book·Ruth R Finkelstein, Christopher D Rock
Jul 15, 2015·International Journal of Molecular Sciences·Chae Woo LimSung Chul Lee
Jun 25, 2004·Plant & Cell Physiology·Xiu-Ping GaoDa-Peng Zhang
Dec 31, 2003·The Journal of Biological Chemistry·Fawzi A RazemRobert D Hill
Sep 20, 2011·Plant, Cell & Environment·Sung Chul Lee, Sheng Luan
Apr 19, 2003·Physiologia Plantarum·Chang-Qing DuanDa-Peng Zhang
Jun 14, 2008·Journal of Experimental Botany·Jiangwei ZhangShangwu Chen
Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Santina BruzzoneElena Zocchi
Jan 13, 2006·Plant Physiology·Xiang-Chun YuDa-Peng Zhang
Sep 16, 2003·Current Opinion in Plant Biology·Axel HimmelbachErwin Grill
May 4, 2004·Plant Physiology·Ling-Yun ZhangDa-Peng Zhang
May 3, 2005·Annual Review of Plant Biology·Eiji Nambara, Annie Marion-Poll

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.