Absence of a CD34- hematopoietic precursor population in recipients of CD34+ stem cell transplantation

Bone Marrow Transplantation
S KatoTomomitsu Hotta

Abstract

The purified CD34(+) cell fraction has been used for hematopoietic stem cell transplantation since they were demonstrated to have long-term reconstituting ability. Therefore, the potential effects of CD34(-) stem cells on the clinical course have been a major concern in recipients of CD34(+)-selected transplantation. To address this concern, we used an in vitro assay to determine whether transplant recipients have CD34(-)precursor population. Lin(-)CD34(-) cells were isolated from bone marrow cells in 11 transplant recipients including four CD34-selected transplantations, six standard bone marrow transplantations, and one T cell-depleted marrow transplantation. The frequency of the Lin(-)CD34(-) population in four CD34-enriched transplantation recipients was not different from those of normal donors or recipients of other modes of transplantation: 0.96 +/- 1.01% (mean +/- s.d., n = 4), 0.45 +/- 0.16% (n = 6), and 0.66 +/- 0.59% (n = 7), respectively. However, the Lin(-)CD34(-)population obtained from the recipients of CD34-enriched transplantation acquired neither CD34 expression nor colony-forming activity after 7 days of culture, whereas the cells from all the normal individuals and standard BMT recipients were able to differ...Continue Reading

References

Mar 1, 1988·The Journal of Clinical Investigation·R J BerensonI D Bernstein
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·M BhatiaJ E Dick
Sep 8, 1999·Science·B L ZieglerC Peschle
May 3, 2000·British Journal of Haematology·Y NakamuraS Kato

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