Absence of anionic phospholipids in Kluyveromyces lactis cells is fatal without F1-catalysed ATP hydrolysis

Canadian Journal of Microbiology
Viktoria PalovicovaM Obernauerová

Abstract

We have shown in previous research that the loss of phosphatidylglycerol and cardiolipin caused by disruption of the PGS1 gene is lethal for the petite-negative yeast Kluyveromyces lactis . This present study demonstrates the role and mechanism of atp2.1 in the suppression of pgs1 lethality in K. lactis cells. Phenotypic characterization has shown that a strain lacking the phosphatidylglycerolphosphate synthase (atp2.1pgs1Δ) possessed a markedly impaired respiratory chain, very low endogenous respiration, and uncoupled mitochondria. As a result the mutant strain was unable to generate a sufficient mitochondrial membrane potential via respiration. The atp2.1 suppressor mutation enabled an increase in the affinity of F(1)-ATPase for ATP in the hydrolytic reaction, resulting in the maintenance of sufficient membrane potential for the biogenesis of mitochondria and survival of cells lacking anionic phospholipid biosynthesis.

References

Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·G S GrootG Schatz
Jan 1, 1995·Methods in Enzymology·R H LawP Nagley
Apr 1, 1996·Nature Genetics·S BioneD Toniolo
Nov 1, 1996·Genetics·A MochizukiY Iwasa
Oct 28, 1996·Molecular & General Genetics : MGG·G D Clark-Walker, X J Chen
Apr 19, 2000·The Biochemical Journal·V Koshkin, M L Greenberg
May 4, 2002·The Biochemical Journal·Vasilij Koshkin, Miriam L Greenberg
Jan 18, 2003·Biochimica Et Biophysica Acta·Jeanie B McMillin, William Dowhan
Oct 17, 2003·The Journal of Biological Chemistry·Kathy PfeifferHermann Schägger
Nov 26, 2004·Molecular Biology of the Cell·Quan ZhongMiriam L Greenberg
Jul 1, 1993·Applied and Environmental Microbiology·M SánchezA Domínguez
Jan 25, 2007·The Journal of Biological Chemistry·Yamin WangDavid M Mueller
Dec 14, 2007·Journal of Lipid Research·Michael Schlame
Apr 24, 2008·Molecular Microbiology·Shuliang ChenMiriam L Greenberg

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