Abstract
Bone sialoprotein (BSP) is highly expressed in early bone deposition and may play a part in primary bone mineralization. We previously showed that while BSP−/− mice have a mild secondary bone phenotype and are responsive to mechanical (unloading) and hormonal (ovariectomy, parathyroid hormone (PTH)) challenges, repair of a cortical bone defect, which involves primary bone deposition is significantly delayed in these mice. In the present study, we investigated the role of BSP in a pure model of primary bone modeling. Bone marrow was ablated by trans-epiphysis aspiration in the femora of BSP+/+ and BSP−/− mice, and 7 days post surgery μCT analysis showed vigorous new bone formation in the shaft of BSP+/+ animals but much less in BSP−/− mice. After 14 days, the volume of medullary bone was significantly decreased as expected in BSP+/+ mice, while it remained stable in the BSP−/−. Osteoid thickness and surface were higher in BSP−/− at day 7, suggesting delayed mineralization, while osteoclast surface and number were significantly lower at day 14, a stage of high medullary bone resorption. At day 7, mRNA expression of early osteoblast marker genes (RUNX2, osterix, alkaline phosphatase, osteopontin) did not differ between the two gen...Continue Reading
References
May 1, 1969·The Anatomical Record·S AmselW H Crosby
Jan 1, 1994·The Histochemical Journal·J ChenJ Sodek
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A NagyJ C Roder
May 29, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J P Gorski
Dec 30, 1999·Endocrinology·T YamashitaM Noda
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B GanssJ Sodek
Mar 13, 2003·Journal of Electron Microscopy·Yoshiko SawaeTakahisa Sasaki
May 2, 2003·Calcified Tissue International·X-D ChenM Young
Sep 4, 2003·Connective Tissue Research·Larry W Fisher, Neal S Fedarko
Sep 19, 2003·Bone·Charles A FrolikJanet M Hock
Mar 9, 2004·The Journal of Biological Chemistry·Jeff P GorskiRonald J Midura
Apr 28, 2004·The Journal of Craniofacial Surgery·Troels T Andreassen, Vittorio Cacciafesta
Jun 19, 2007·Bone·Jonathan A R GordonHarvey A Goldberg
Jun 16, 2009·Bone·Luc MalavalJoëlle Amédée
Sep 19, 2009·Bone·Laurent MonfouletOlivier Chassande
Sep 3, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Maya BoudiffaLuc Malaval
Sep 17, 2010·Endocrinology·Ndéye Marième Wade-GueyeLuc Malaval
Citations
Mar 28, 2013·Cell and Tissue Research·Yanping WangZhipeng Fan
Jan 31, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Ryan MarinovichHarvey A Goldberg
Jan 15, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Wafa BouleftourLuc Malaval
Jul 19, 2015·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Juliana Medeiros de CamposRenato Corrêa Viana Casarin
Mar 21, 2013·Cell Proliferation·L WangH Liao
Oct 10, 2013·Oral Diseases·Y WangJ Yu
Oct 25, 2013·Cell Proliferation·Y WangJ Yu
Dec 17, 2014·Journal of Cellular Physiology·Renata Neves GranitoLuc Malaval
May 28, 2013·Experimental Cell Research·Victoria B Ayala-PeñaGraciela E Santillán
May 13, 2015·Bone·B L FosterM J Somerman
Nov 19, 2014·Stem Cell Research & Therapy·Yao LiJinhua Yu
Sep 16, 2014·Biochimica Et Biophysica Acta·Klaus Jung, Michael Lein
Feb 25, 2015·PloS One·Guenaelle BouetLuc Malaval
Jun 16, 2015·PloS One·Jin Woo LeeDongwoo Khang
Oct 6, 2016·Journal of Cellular Physiology·Wafa BouleftourLuc Malaval
Jun 27, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Wei XuXiaolan Du
Nov 22, 2017·Oncotarget·Zhi-Qi WuHua-Guo Xu
Feb 28, 2014·International Journal of Molecular Medicine·Woon-Won Jung
Jul 23, 2019·Journal of Oral Science·Ting WeiTingsong Liu
Oct 6, 2017·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·William Marcatti Amarú MaximianoConstance Oliver
Apr 6, 2018·Frontiers in Bioengineering and Biotechnology·Behzad JavaheriAndrew A Pitsillides
Nov 25, 2018·Materials·Andreas BaranowskiAlexander Hofmann
May 16, 2019·Stem Cells International·Guanqi LiuHong Ai
Aug 8, 2021·International Journal of Molecular Sciences·Icela Palma-LaraCarmen Palacios-Reyes