PMID: 22586700May 16, 2012Paper

Absence of bone sialoprotein (BSP) impairs primary bone formation and resorption: the marrow ablation model under PTH challenge

Bone
Ndéyé Marième Wade-GuéyeLuc Malaval

Abstract

Bone sialoprotein (BSP) is highly expressed in early bone deposition and may play a part in primary bone mineralization. We previously showed that while BSP−/− mice have a mild secondary bone phenotype and are responsive to mechanical (unloading) and hormonal (ovariectomy, parathyroid hormone (PTH)) challenges, repair of a cortical bone defect, which involves primary bone deposition is significantly delayed in these mice. In the present study, we investigated the role of BSP in a pure model of primary bone modeling. Bone marrow was ablated by trans-epiphysis aspiration in the femora of BSP+/+ and BSP−/− mice, and 7 days post surgery μCT analysis showed vigorous new bone formation in the shaft of BSP+/+ animals but much less in BSP−/− mice. After 14 days, the volume of medullary bone was significantly decreased as expected in BSP+/+ mice, while it remained stable in the BSP−/−. Osteoid thickness and surface were higher in BSP−/− at day 7, suggesting delayed mineralization, while osteoclast surface and number were significantly lower at day 14, a stage of high medullary bone resorption. At day 7, mRNA expression of early osteoblast marker genes (RUNX2, osterix, alkaline phosphatase, osteopontin) did not differ between the two gen...Continue Reading

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Citations

Jan 31, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Ryan MarinovichHarvey A Goldberg
Jan 15, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Wafa BouleftourLuc Malaval
Jul 19, 2015·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Juliana Medeiros de CamposRenato Corrêa Viana Casarin
Dec 17, 2014·Journal of Cellular Physiology·Renata Neves GranitoLuc Malaval
Jun 27, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Wei XuXiaolan Du
Feb 28, 2014·International Journal of Molecular Medicine·Woon-Won Jung
Oct 6, 2017·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·William Marcatti Amarú MaximianoConstance Oliver
Apr 6, 2018·Frontiers in Bioengineering and Biotechnology·Behzad JavaheriAndrew A Pitsillides
Aug 8, 2021·International Journal of Molecular Sciences·Icela Palma-LaraCarmen Palacios-Reyes

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