Absence of Bsep/Abcb11 attenuates MCD diet-induced hepatic steatosis but aggravates inflammation in mice.

Liver International : Official Journal of the International Association for the Study of the Liver
Claudia D FuchsMichael Trauner

Abstract

Bile acids (BAs) regulate hepatic lipid metabolism and inflammation. Bile salt export pump (BSEP) KO mice are metabolically preconditioned with a hydrophilic BA composition protecting them from cholestasis. We hypothesize that changes in hepatic BA profile and subsequent changes in BA signalling may critically determine the susceptibility to steatohepatitis. Wild-type (WT) and BSEP KO mice were challenged with methionine choline-deficient (MCD) diet to induce steatohepatitis. Serum biochemistry, lipid profiling as well as intestinal lipid absorption were assessed. Markers of inflammation, fibrosis, lipid and BA metabolism were analysed. Hepatic and faecal BA profile as well as serum levels of the BA synthesis intermediate 7-hydroxy-4-cholesten-3-one (C4) were also investigated. Bile salt export pump KO MCD-fed mice developed less steatosis but more inflammation than WT mice. Intestinal neutral lipid levels were reduced in BSEP KO mice at baseline and under MCD conditions. Faecal non-esterified fatty acid concentrations at baseline and under MCD diet were markedly elevated in BSEP KO compared to WT mice. Serum liver enzymes and hepatic expression of inflammatory markers were increased in MCD-fed BSEP KO animals. PPARα protein le...Continue Reading

References

Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S A KliewerJ M Lehmann
May 23, 1998·The Journal of Biological Chemistry·T GerloffP J Meier
May 21, 1999·Science·M MakishimaB Shan
May 21, 1999·Science·D J ParksJ M Lehmann
Jun 9, 1999·Molecular Cell·H WangB M Forman
May 10, 2002·Journal of Gastroenterology and Hepatology·Paul Angulo, Keith D Lindor
Oct 31, 2002·Gastroenterology·Arun J Sanyal, UNKNOWN American Gastroenterological Association
Oct 31, 2002·Best Practice & Research. Clinical Gastroenterology·C P Day
Apr 16, 2003·Alimentary Pharmacology & Therapeutics·L M Alba, K Lindor
Oct 23, 2003·The Journal of Biological Chemistry·Anne FiggeRichard M Green
Jan 15, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shikha S SundaramRichard M Green
May 4, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kanji YamaguchiAnna Mae Diehl
Feb 19, 2008·Journal of Lipid Research·Vitali MatyashDominik Schwudke
Oct 31, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yan-Dong WangWendong Huang
Oct 31, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Martin WagnerMichael Trauner
Nov 6, 2009·European Journal of Epidemiology·Gabriella AndreottiAnn W Hsing
Dec 2, 2010·Nature Reviews. Gastroenterology & Hepatology·Lionel Hebbard, Jacob George
Dec 21, 2010·Bioinformatics·Jürgen HartlerGerhard G Thallinger
Dec 3, 2011·Current Opinion in Gastroenterology·Karen D Corbin, Steven H Zeisel
Feb 1, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Naoki TanakaFrank J Gonzalez
May 9, 2012·Cellular Signalling·Zhizhen XuFengtian He
Nov 2, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Naoki Tanaka, Frank J Gonzalez
May 31, 2013·The Journal of Biological Chemistry·Harmeet MalhiRandal J Kaufman
Sep 5, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Pooja JhaMichael Trauner
Mar 7, 2014·Biochimica Et Biophysica Acta·Pooja JhaMichael Trauner
Nov 1, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Naoki Tanaka, Frank J Gonzalez
Mar 13, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Charles P NajtBarbara P Atshaves
Sep 7, 2016·Journal of Hepatology·Claudia D FuchsMichael Trauner
Apr 18, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Alexander TrieblHarald C Köfeler
Jul 12, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Puneet PuriArun J Sanyal
Jul 27, 2017·Liver International : Official Journal of the International Association for the Study of the Liver·Valerio NobiliChristopher D Byrne

❮ Previous
Next ❯

Citations

Feb 5, 2021·Scientific Reports·Meng-Yu ZhangXian-Ming Xia
Mar 14, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Jonathan A ShepsVictor Ling

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR
antisense oligonucleotide
dissection

Software Mentioned

Lipid Data Analyzer

Related Concepts

Related Feeds

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.