PMID: 7515252Jan 1, 1994Paper

Absence of changes in metallothionein RNA in the rat testes made refractory to cadmium toxicity by zinc pretreatment

Human & Experimental Toxicology
Z Z WahbaM P Waalkes

Abstract

Testicular toxicity and interstitial cell tumours induced by cadmium are prevented by zinc or by low dose cadmium pretreatments. The mechanism of this tolerance is unknown, though metallothionein (MT) is thought to play a role in tissue resistance to cadmium toxicity. Thus, the possible involvement of the testicular MT gene in metal-induced tolerance to cadmium toxicity was studied. Rats were pretreated with zinc (1.0 mmol kg-1, s.c.). Histological examination of the testes indicated such pretreatments prevented the necrotizing effects of subsequent doses of cadmium (20 mumol kg-1, s.c.) administered 24 h later. RNA was extracted from testes or liver 24 h after zinc pretreatment, and analysed by the slot blot technique using the p2A10 cDNA probe to the MT gene. Zinc pretreatment had little effect on MT RNA in the testes, and such pretreatments did not alter testicular cadmium-binding protein capacity. In contrast, RNAs derived from livers of zinc pretreated rats showed marked increases in MT RNA and MT protein. Hence, the testicular MT gene does not appear to play a major role in the induced tolerance to cadmium toxicity and carcinogenesis generated by zinc.

References

Nov 1, 1992·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M P Waalkes, S Rehm
Jan 1, 1987·Methods in Enzymology·R J MacDonaldJ M Chirgwin
Jan 1, 1984·Journal of Toxicology and Environmental Health·P L Goering, C D Klaassen
Jul 1, 1984·Toxicology and Applied Pharmacology·P L Goering, C D Klaassen

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Citations

Oct 29, 2000·Archives of Biochemistry and Biophysics·T AbeK Higashi
May 30, 2009·Archives of Toxicology·Hideaki ShimadaYorishige Imamura
Apr 20, 2011·Archives of Environmental Contamination and Toxicology·Haiquan ZhaoFashui Hong
Aug 29, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M M Brzóska, J Moniuszko-Jakoniuk
Feb 4, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Elzbieta BondaAlicja Krasowska
Aug 21, 2010·Journal of Hazardous Materials·Yaling CuiFashui Hong
Sep 17, 2003·The Journal of Toxicological Sciences·Takashi AshinoTakemi Yoshida

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