Absence of cyclic adenosine 3':5' monophosphate responsive element modulator expression at the spermatocyte arrest stage

Fertility and Sterility
W W LinE D Kim

Abstract

To test the hypotheses that variations in the expression of adenosine 3':5' monophosphate (cAMP) responsive element modulator are found in human seminiferous epithelium in men with impaired testicular function and subsequent infertility and that variations in apoptosis frequency are associated with differential cAMP responsive element modulator expression in male infertility states. Standard immunohistochemical staining using a rabbit polyclonal antibody against the tau isoform of the cAMP responsive element modulator protein was performed on 5-microM sections of Bouin's fixed, paraffin-embedded testicular tissue obtained from azoospermic or severely oligozoospermic men for routine clinical purposes. Histologic diagnosis was confirmed with computerized image analysis of Feulgen-stained sections. Tertiary male infertility referral center at a medical school. Forty-eight testis biopsies were performed in 38 azoospermic or severely oligozoospermic males. Rabbit polyclonal cAMP responsive element modulator tau antibody was applied to the paraffin-embedded testis sections. Testis immunoreactivity to polyclonal cAMP responsive element modulator tau antibody and apoptotic indices. Although cAMP responsive element modulator immunoreact...Continue Reading

References

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Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F A van der Hoorn, H A Tarnasky
Jul 1, 1997·The Journal of Urology·E D KimL I Lipshultz

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Citations

Feb 9, 1999·Prenatal Diagnosis·E D KimD J Lamb
Jun 1, 2012·Journal of Assisted Reproduction and Genetics·Mehdi TotonchiHamid Gourabi
Jun 17, 2000·Fertility and Sterility·W Y SonY C Kim
Jun 21, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Anna HentrichLutz Konrad
Jul 7, 2010·Genetics and Molecular Research : GMR·L K PandeyA K Saxena
Feb 12, 2005·Reproductive Biomedicine Online·Csilla Krausz, Paolo Sassone-Corsi
Jan 9, 2003·The Urologic Clinics of North America·Edward D KimRandall B Meacham
Dec 1, 2000·Baillière's Best Practice & Research. Clinical Endocrinology & Metabolism·S BhasinK Ma
Jul 11, 2006·Human Fertility : Journal of the British Fertility Society·Kevin N Hogeveen, Paolo Sassone-Corsi
Nov 18, 2000·Journal of Endocrinological Investigation·A Peri, M Serio

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis