Absence of detectable benzimidazole-resistance associated alleles in Haemonchus placei in cattle in Nigeria revealed by pyrosequencing of β-tubulin isotype 1

Parasitology Research
Isaiah O AdemolaG von Samson-Himmelstjerna

Abstract

Trichostrongyles are gastrointestinal parasites that occur globally and can cause subclinical to severe, sometimes life-threatening, infections in ruminants, particularly young animals. Benzimidazoles (BZ) are commonly used for the treatment of gastrointestinal parasites in ruminants. Increasing spread of worm populations with anthelmintics resistance has been reported and is considered a consequence of highly frequent and longstanding use of anthelmintics. To obtain initial information regarding the occurrence of putatively BZ-resistant Nigerian Haemonchus populations, screening based on the molecular analysis of BZ-resistance-associated β-tubulin isotype 1 gene sequence polymorphisms was undertaken. Genomic DNA was isolated from pooled adult Haemonchus sp. from 35 animals from each of the six states of southwestern Nigeria. Sequencing of internal transcribed spacer 2 (ITS-2) and external transcribed spacer (ETS) regions was used to determine the Haemonchus species. Pyrosequencing assays were used for detection of single-nucleotide polymorphisms (SNPs) in the β-tubulin isotype 1 genes of the worms at codons 200 and 167 (TTC/TAC) or 198 (GAA/GCA). Exclusively, Haemonchus placei was detected and allele frequencies obtained at al...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 15, 2004·Trends in Parasitology·Adrian J WolstenholmeNicholas C Sangster
Apr 23, 2005·Veterinary Parasitology·M A Alvarez-SánchezF A Rojo-Vázquez
Jan 19, 2006·Veterinary Parasitology·Georg von Samson-Himmelstjerna
Jan 24, 2006·Veterinary Parasitology·G C ColesJ Vercruysse
Mar 29, 2006·Veterinary Parasitology·J TiwariK M L Pathak
Jun 12, 2014·Veterinary Parasitology·Umer ChaudhryJohn Gilleard

❮ Previous
Next ❯

Citations

Nov 18, 2016·Scientific Reports·Tongjit ThanchomnangWanchai Maleewong
Feb 10, 2021·Parasites & Vectors·Khalid M MohammedsalihGeorg von Samson-Himmelstjerna

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.