PMID: 702657Oct 1, 1978Paper

Absence of detectable capping and methylating enzymes in influenza virions

Journal of Virology
S J PlotchR M Krug

Abstract

In the presence of Mg(2+) and a specific dinucleotide primer (ApG or GpG), the influenza virion transcriptase synthesizes the eight discrete segments of complementary RNA (cRNA) containing polyadenylic acid (Plotch and Krug, J. Virol. 21:24-34, 1977). Virions were examined for their ability to cap and methylate cRNA containing di- or triphosphorylated 5' termini. By using the primers ppApG, pppApG, or ppGpG, viral cRNA was synthesized in vitro with [alpha-(32)P]-GTP and S-[methyl-(3)H]adenosylmethionine as labeled precursors. DEAE-Sephadex chromatography of the RNase T2 digest of the cRNA product demonstrated no (3)H incorporation at all and the absence of a (32)P-labeled cap structure. The 5' terminus of ppApG-primed cRNA could be capped and methylated by enzymes from vaccinia virus, indicating that the two 5'-terminal phosphates derived from the primer were preserved in the product cRNA. The cap structure formed by the vaccinia enzymes and released by RNase T2 digestion as m(7)GpppA(m)pGp was radioactively labeled at its 3'-terminal phosphate only when [alpha-(32)P]CTP was used as the labeled precursor during transcription. This indicates that the 5'-terminal sequence of the cRNA is ppApGpC and that, therefore, ppApG most pro...Continue Reading

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Citations

Jan 1, 1980·Annals of the New York Academy of Sciences·C J LaiR M Chanock
Mar 16, 2011·Virology Journal·Olive T W Li, Leo L M Poon
Oct 30, 2018·American Journal of Respiratory Cell and Molecular Biology·Kambez H BenamLing-Pei Ho
Jan 6, 2021·Briefings in Functional Genomics·Matthew T Sacco, Stacy M Horner
Mar 27, 2021·Annual Review of Biochemistry·Tim KrischunsStephen Cusack
Jun 1, 1980·Microbiological Reviews·A K Banerjee

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