Absence of equilibrium cluster phase in concentrated lysozyme solutions.

Proceedings of the National Academy of Sciences of the United States of America
Anuj ShuklaD I Svergun

Abstract

In colloidal systems, the interplay between the short range attraction and long-range repulsion can lead to a low density associated state consisting of clusters of individual particles. Recently, such an equilibrium cluster phase was also reported for concentrated solutions of lysozyme at low ionic strength and close to the physiological pH. Stradner et al. [(2004) Equilibrium cluster formation in concentrated protein solutions and colloids. Nature 432:492-495] found that the position of the low-angle interference peak in small-angle x-ray and neutron scattering (SAXS and SANS) patterns from lysozyme solutions was essentially independent of the protein concentration and attributed these unexpected results to the presence of equilibrium clusters. This work prompted a series of experimental and theoretical investigations, but also revealed some inconsistencies. We have repeated these experiments following the protein preparation protocols of Stradner et al. using several batches of lysozyme and exploring a broad range of concentrations, temperature and other conditions. Our measurements were done in multiple experimental sessions at three different high-resolution SAXS and SANS instruments. The low-ionic-strength lysozyme soluti...Continue Reading

References

Oct 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·L A KiesowJ B Shelton
Jan 25, 1974·Journal of Molecular Biology·R Diamond
May 23, 1972·Biochemistry·C Tanford, R Roxby
Jun 1, 1996·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·M L BroideM D Saxowsky
Apr 17, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·K DawsonE Zaccarelli
Jun 21, 2001·Physical Review Letters·P N SegrèD A Weitz
Nov 3, 2001·Physical Review Letters·M BoströmB W Ninham
Mar 23, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Giuseppe FoffiGeorge Stell
Mar 18, 2003·Faraday Discussions·Amit M KulkarniCharles F Zukoski
Jun 6, 2003·Physical Review Letters·D PontoniD Beysens
Nov 27, 2004·Nature·Anna StradnerPeter Schurtenberger
Mar 3, 2005·The Journal of Chemical Physics·Yun LiuSow-Hsin Chen
Jul 23, 2005·The Journal of Chemical Physics·Luis F FilobeloPeter G Vekilov
Aug 11, 2005·Physical Review Letters·Andrew I CampbellPaul Bartlett
Oct 20, 2006·The Journal of Physical Chemistry. B·Anna StradnerPeter Schurtenberger
Feb 7, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·M SztuckiH Hoekstra
Aug 7, 2007·Physical Review Letters·Nadeem JavidRoland Winter

❮ Previous
Next ❯

Citations

Jan 1, 1990·Cellular Signalling·G R GuyC S Peng
Dec 15, 1986·Biochemical and Biophysical Research Communications·J SeidlI Ziegler
Dec 18, 2013·Journal of the American Chemical Society·John T KingKevin J Kubarych
Jun 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Sugam KumarJoachim Kohlbrecher
Jul 7, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Bhuvnesh BhartiGerhard H Findenegg
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jill Trewhella
Nov 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·A StradnerP Schurtenberger
Mar 7, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Peter G Vekilov, Maria A Vorontsova
Jan 10, 2012·The Journal of Chemical Physics·Maria Grazia OrtoreFrancesco Spinozzi
Apr 14, 2013·The Journal of Chemical Physics·J PȩkalskiN G Almarza
Oct 29, 2013·The Journal of Chemical Physics·P Douglas GodfrinNorman J Wagner
Feb 22, 2011·Annual Review of Biophysics·Lois Pollack
Feb 22, 2016·The Journal of Chemical Physics·Debra J AudusJack F Douglas
Aug 8, 2008·Journal of Pharmaceutical Sciences·William F WeissChristopher J Roberts
Feb 5, 2015·Biophysical Journal·Prasad S SarangapaniJai A Pathak
May 25, 2010·Biophysical Chemistry·Sergey P Rozhkov, Andrey S Goryunov
Jan 2, 2016·The Journal of Physical Chemistry Letters·P S AlvesM S Rocha
Nov 5, 2015·Biophysical Journal·Maria A VorontsovaPeter G Vekilov
Sep 2, 2009·Biophysical Journal·Bankanidhi SahooSudipta Maiti
Feb 25, 2014·Biophysical Journal·David P Goldenberg, Brian Argyle
May 13, 2015·Journal of Chromatography. a·Stijn H S KoshariAbraham M Lenhoff
Nov 30, 2010·Biophysical Journal·Paolo MereghettiRebecca C Wade
Jul 20, 2016·Journal of the American Chemical Society·Matthias RoosKay Saalwächter
Oct 21, 2016·Physical Chemistry Chemical Physics : PCCP·Coralie PasquierStéphane Pezennec
Apr 17, 2015·Faraday Discussions·Zoubida HammadiStéphane Veesler
Jan 10, 2018·Reports on Progress in Physics·Joel BerryMikko Haataja
Oct 7, 2017·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Fajun Zhang
Sep 23, 2014·Journal of Applied Crystallography·Magda SkouRichard E Gillilan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Jill Trewhella
The Journal of Chemical Physics
Yun LiuSow-Hsin Chen
© 2022 Meta ULC. All rights reserved