Absence of Fer protein tyrosine kinase exacerbates endotoxin induced intestinal epithelial barrier dysfunction in vivo

W QiD-M McCafferty


Fer kinase is activated by a number of growth factors and cytokines, and phosphorylates cortactin during cell shape change induced cortical actin reorganisation. In addition, Fer participates in cytoskeletal interactions mediated by cadherins, platelet endothelial cell adhesion molecule 1 (PECAM-1), and integrins, and has recently been implicated in limiting the innate immune response. Here we examined the role of Fer in modulating leucocyte recruitment and epithelial barrier function in the gut in response to lipopolysaccharide (LPS). Mice targeted with a kinase inactivating mutation (FerDR) or strain matched wild-type (129Sv/J) mice were studied after intraperitoneal injection of LPS. Intravital microscopy was used to examine intestinal leucocyte kinetics, and leucocyte infiltration was assessed by fluorescence activated cell sorting. Systemic inflammation was assessed by measuring lung myeloperoxidase activity. Epithelial barrier function was assessed in vivo using blood to lumen 51Cr-EDTA clearance, with or without antibody based depletion of circulating neutrophils. LPS induced a significant increase in leucocyte adhesion and neutrophil infiltration into the intestinal tissue, and increased blood to lumen 51Cr-EDTA clearan...Continue Reading


Oct 1, 1992·Inflammation·H ArndtD N Granger
Dec 15, 1991·Klinische Wochenschrift·H ArndtD N Granger
Sep 1, 1987·Reviews of Infectious Diseases·H S Warren, L A Chedid
Aug 26, 1998·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·S MassbergK Messmer
Dec 15, 2000·Oncogene·D R RobinsonS F Lin
Aug 23, 2002·Molecular and Cellular Biology·Andrew W B Craig, Peter A Greer
Feb 20, 2003·Science's STKE : Signal Transduction Knowledge Environment·Bryan Heit, Paul Kubes
Apr 12, 2003·FEBS Letters·Denise E Jackson
Jun 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jacqueline R McDermottRichard K Grencis
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·Y A SenisP A Greer
Aug 21, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Matthias MaasPeter J Newman
Oct 27, 2004·Experimental Hematology·Waheed SangrarPeter A Greer

❮ Previous
Next ❯


Jan 9, 2007·Inflammatory Bowel Diseases·Oscar Pastor RojoAgustín Albillos Martínez
Feb 1, 1991·Research in Immunology·L PezziC Martinez
Apr 1, 1997·Critical Reviews in Oncology/hematology·T Tanaka
Jan 10, 2013·Critical Reviews in Microbiology·Andre G Buret, Amol Bhargava
Feb 12, 2010·Expert Opinion on Therapeutic Targets·Pedro Pimentel-NunesAdelino F Leite-Moreira
Dec 24, 2014·The Lancet. Respiratory Medicine·Anna RautanenUNKNOWN ESICM/ECCRN GenOSept Investigators
Mar 12, 2011·Journal of Leukocyte Biology·Maitham KhajahDonna-Marie McCafferty
Jan 29, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maitham KhajahDonna-Marie McCafferty
Sep 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cynthia L LeaphartDavid J Hackam

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.