PMID: 7539624Apr 1, 1995Paper

Absence of G-CSF receptors and absent response to G-CSF in childhood Burkitt's lymphoma and B-ALL cells

British Journal of Haematology
J TomeczkowskiK W Sykora

Abstract

The expression of the granulocyte colony-stimulating factor (G-CSF) receptor in childhood Burkitt's lymphoma (BL) cells, and the mitogenic effect of G-CSF on these cells, was studied in a panel of 13 Epstein-Barr virus (EBV) positive and negative BL cell lines derived from nine children. G-CSF receptor mRNA expression was investigated by Northern blot analysis and reverse transcriptase polymerase chain reaction (RT-PCR). Binding of G-CSF to BL cell lines was measured by chemical crosslinking of 125I-G-CSF, and proliferation by thymidine incorporation. Inducibility of the G-CSF receptor was studied by stimulation with interleukin-1 beta, tumour necrosis factor-alpha, Staphylococcus aureus Cowan A, anti-human IgM, phorbol myristate acetate, calcium ionophore A23187, and by infection in vitro by immortalizing and non-immortalizing strains of EBV. BL cell lines, unstimulated or stimulated by biological reagents or EBV infection, did not bind radioionated G-CSF in crosslinking experiments. No stimulation by recombinant human G-CSF was observed in 3H-thymidine incorporation assays. No G-CSF receptor mRNA was detected by Northern blot analysis or RT-PCR in BL cell lines. It is concluded that G-CSF plays no direct stimulatory role in t...Continue Reading

References

Jan 1, 1992·Journal of Nutritional Science and Vitaminology·C G BegleyW Sheridan
Mar 1, 1991·The Journal of Clinical Investigation·F BussolinoA Mantovani
Apr 1, 1991·European Journal of Haematology·S KondoY Niho
Nov 1, 1989·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J NeidhartM Downing
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H UzumakiY Umezawa
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A CalenderG M Lenoir
Sep 1, 1987·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·F R AppelbaumJ E Sanders
Jan 15, 1987·International Journal of Cancer. Journal International Du Cancer·C G BegleyN A Nicola
Jan 1, 1985·Haematology and Blood Transfusion·E PlatzerM A Moore
Nov 1, 1982·Cancer Genetics and Cytogenetics·R Berger, A Bernheim
Feb 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A ReiterH Riehm

❮ Previous
Next ❯

Citations

Jan 1, 1997·Pediatric Hematology and Oncology·D Schuler
Jan 31, 2004·Pediatric Blood & Cancer·Csongor KissPéter Kovács
May 6, 2009·Genes, Chromosomes & Cancer·Kajsa Paulsson, Bertil Johansson
Aug 27, 2013·Particle & Particle Systems Characterization : Measurement and Description of Particle Properties and Behavior in Powders and Other Disperse Systems·Rekha RaghunathanWilliam E Haskins
Jul 9, 2002·British Journal of Haematology·Keiko MorikawaToshio Miyawaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.