Absence of Gcn5 HAT activity defines a novel state in the opening of chromatin at the PHO5 promoter in yeast

Molecular Cell
P D GregoryWolfram Hörz

Abstract

Histone acetyltransferase (HAT) activity has been demonstrated for several transcriptional activators, formally connecting chromatin modification with gene regulation. However, no effect on chromatin has been demonstrated. We have investigated the role of the HAT Gcn5 at the nucleosomally regulated PHO5 promoter. Under conditions of constitutive submaximal activation (i.e., in the absence of the negative regulator Pho80), deletion of Gcn5 determines a novel randomized nucleosomal organization across the promoter and leads to a dramatic reduction in activity. Furthermore, mutation of amino acids critical for Gcn5 HAT activity is sufficient to generate this structure. This intermediate state in chromatin opening gives way to the fully open structure upon maximal induction (phosphate starvation), even in the absence of Gcn5. Thus, Gcn5 is shown to affect directly the remodeling of chromatin in vivo.

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Citations

Sep 25, 1999·Journal of Cellular Physiology·A Giordano, M L Avantaggiati
Mar 17, 2004·Biochimica Et Biophysica Acta·Hans Reinke, Wolfram Hörz
Jun 26, 1998·Current Opinion in Cell Biology·P D Gregory, W Hörz
Jul 8, 1999·Current Opinion in Cell Biology·S L Berger
Nov 21, 2000·Trends in Biochemical Sciences·J D Aalfs, R E Kingston
Jan 19, 2000·Trends in Biochemical Sciences·C E BrownJ L Workman
Feb 24, 2006·Nature Structural & Molecular Biology·Walter J JessenMichael P Kladde
Jun 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christopher D CarvinMichael P Kladde
Dec 26, 2006·The Journal of Biological Chemistry·Stephanie A MorrisBrian D Strahl
Mar 18, 2008·Nucleic Acids Research·K H Brettingham-MooreA F Holloway
Jun 8, 2011·Molecular and Cellular Biology·Shan-Shan WangJin-Qiu Zhou
Dec 28, 1999·Molecular and Cellular Biology·R BelotserkovskayaS L Berger
May 2, 2003·Molecular and Cellular Biology·Slobodan BarbaricWolfram Hörz
May 16, 2003·Molecular and Cellular Biology·Daniel W Neef, Michael P Kladde
Dec 2, 2004·Molecular and Cellular Biology·Philipp KorberWolfram Hörz
Mar 16, 2005·Molecular and Cellular Biology·Archana Dhasarathy, Michael P Kladde
Feb 16, 2006·Molecular and Cellular Biology·Rachel Maria ImoberdorfMichel Strubin
Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·D E Sterner, S L Berger
Feb 22, 2012·Genetics·Oliver J Rando, Fred Winston
Mar 8, 2013·Neuromolecular Medicine·Bess M FlashnerG Ian Gallicano
Nov 18, 2010·Molecular Systems Biology·Changhui MaoHinrich Boeger
May 2, 2003·Annals of the New York Academy of Sciences·Fyodor D Urnov
Mar 31, 2009·Yeast·Rhiannon Biddick, Elton T Young
Apr 17, 2001·Experimental Cell Research·P D GregoryW Hörz

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