Absence of Global Stress Regulation in Escherichia coli Promotes Pathoadaptation and Novel c-di-GMP-dependent Metabolic Capability

Scientific Reports
Nikola Zlatkov, Bernt Eric Uhlin

Abstract

Pathoadaptive mutations linked to c-di-GMP signalling were investigated in neonatal meningitis-causing Escherichia coli (NMEC). The results indicated that NMEC strains deficient in RpoS (the global stress regulator) maintained remarkably low levels of c-di-GMP, a major bacterial sessility-motility switch. Deletion of ycgG2, shown here to encode a YcgG allozyme with c-di-GMP phosphodiesterase activity, and the restoration of RpoS led to a decrease in S-fimbriae, robustly produced in artificial urine, hinting that the urinary tract could serve as a habitat for NMEC. We showed that NMEC were skilled in aerobic citrate utilization in the presence of glucose, a property that normally does not exist in E. coli. Our data suggest that this metabolic novelty is a property of extraintestinal pathogenic E. coli since we reconstituted this ability in E. coli UTI89 (a cystitis isolate) via deactivation rpoS; additionally, a set of pyelonephritis E. coli isolates were shown here to aerobically use citrate in the presence of glucose. We found that the main reason for this metabolic capability is RpoS inactivation leading to the production of the citrate transporter CitT, exploited by NMEC for ferric citrate uptake dependent on YcgG2 (an alloz...Continue Reading

References

Dec 1, 1978·Journal of General Microbiology·H W SmithP Green
Nov 1, 1979·Applied and Environmental Microbiology·N IshiguroG Sato
Jan 1, 1987·Analytical Biochemistry·B Schwyn, J B Neilands
May 30, 1974·The New England Journal of Medicine·J B RobbinsL A Hanson
Jul 1, 1980·Journal of General Microbiology·M Lütgens, G Gottschalk
Mar 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C M KuninJ Hacker
Mar 1, 1997·Letters in Applied Microbiology·T Brooks, C W Keevil
Jan 1, 1997·International Urology and Nephrology·X GiannakopoulosK Charalambopoulos
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Nov 1, 1959·Journal of Applied Physiology·I C PLOUGH, E M BAKER
Mar 26, 2004·Nature Reviews. Microbiology·James B KaperHarry L Mobley
Aug 20, 2004·Journal of Bacteriology·Thea KingThomas Ferenci
Oct 13, 2004·FEMS Immunology and Medical Microbiology·Yi XieKwang Sik Kim
Oct 27, 2004·Infection and Immunity·Jennifer A SnyderHarry L T Mobley
Oct 22, 2005·International Journal of Medical Microbiology : IJMM·Stéphane Bonacorsi, Edouard Bingen
Jun 6, 2006·Gene·Simanti DattaDonald L Court
Oct 3, 2006·Molecular Microbiology·Harald WeberRegine Hengge
Sep 4, 2008·Genes & Development·Christina PesaventoRegine Hengge
Apr 14, 2010·Journal of Microbiological Methods·Christian SpanglerVolkhard Kaever
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Danilo Gomes MorielLaura Serino
Jun 7, 2011·Annual Review of Microbiology·Aurelia BattestiSusan Gottesman

❮ Previous
Next ❯

Citations

Oct 9, 2020·Biofouling·Fazlurrahman KhanYoung-Mog Kim
Oct 13, 2020·Frontiers in Microbiology·Herb E Schellhorn
Jun 3, 2021·International Journal of Molecular Sciences·Giada AntonelliFrancesca Schiavetti

❮ Previous
Next ❯

Datasets Mentioned

BETA
MG1655
X81838.1
RS218
NA114

Methods Mentioned

BETA
PCR

Software Mentioned

GATK HaplotypeCaller
EMBOSS
BWA mem
SnapGene
GraphPad Prism
Clustal Omega
GATK VariantFiltration

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Laboratory and Clinical Medicine
Dale E Hammerschmidt
Casopís lékar̆ů c̆eských
V PACES
Deutsche medizinische Wochenschrift
R FünfstückK G Naber
Annals of Emergency Medicine
E AbrahamR R Simon
© 2022 Meta ULC. All rights reserved