Absence of IE1 p72 protein function during low-multiplicity infection by human cytomegalovirus results in a broad block to viral delayed-early gene expression

Journal of Virology
Jonathan M Gawn, R F Greaves

Abstract

Human cytomegalovirus (HCMV) ie1 deletion mutant CR208 is profoundly growth deficient after low-multiplicity infection of primary fibroblasts. Previously, we showed that many fewer cells infected with CR208 at low multiplicity accumulated the delayed-early (DE) protein ppUL44 than accumulated the immediate-early 2 (IE2) p86 protein, indicating a high frequency of abortive infections. We now demonstrate that accumulation of all DE proteins tested was defective after low-multiplicity infection in the absence of IE1 p72. Accumulation of the DE proteins pUL57, pUL98, and pUL69 followed a pattern very similar to that of ppUL44 during low-multiplicity CR208 infection. Accumulation of the ppUL112-113 proteins occurred in a greater proportion of cells than other DE proteins during low-multiplicity CR208 infection, but was still deficient relative to wild-type virus. We also show for the first time that steady-state levels of many DE RNAs were reduced during low-multiplicity CR208 infection and that by in situ hybridization of the abundant cytoplasmic 2.7-kb TRL4 DE (beta2.7) RNA, a viral DE RNA followed a defective pattern of accumulation similar to that of ppUL44. Furthermore, transfected DE promoter-reporter constructs were found in ...Continue Reading

References

Aug 1, 1975·Journal of Virology·E S Huang
Jan 1, 1992·Journal of Virology·A M Colberg-PoleyD J Tenney
Jan 1, 1990·Current Topics in Microbiology and Immunology·M S CheeJ A Martignetti
Feb 1, 1987·Molecular and Cellular Biology·J R de WetS Subramani
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L E PostB Roizman
Apr 1, 1982·Gene·B FleckensteinJ Collins
Jan 1, 1984·Journal of Virology·R M StenbergM F Stinski
Dec 1, 1995·Journal of Virology·H ZhuT Shenk

❮ Previous
Next ❯

Citations

Feb 2, 2008·Medical Microbiology and Immunology·Andreas BuscheMartin Messerle
Mar 27, 2003·Journal of Autoimmunity·Marijke Van GhelueOle Petter Rekvig
May 31, 2013·Journal of Virology·Shinya Omoto, Edward S Mocarski
Sep 13, 2008·Journal of Virology·Alexandra NitzscheMichael Nevels
Jul 1, 2006·Intervirology·Diego A RivaSusana E Mersich
Jan 21, 2014·Basic Research in Cardiology·Koon-Chu YaiwCecilia Söderberg-Nauclér
Apr 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eain MurphyArnold J Levine
Dec 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michael NevelsThomas Shenk
Feb 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christina PaulusMichael Nevels
May 17, 2011·Pharmacology & Therapeutics·Beatrice MercorelliArianna Loregian
Feb 14, 2006·Cellular Microbiology·Roger D Everett
Dec 23, 2011·Biology of the Cell·Nina Van OpdenboschGerlinde R Van de Walle
Dec 11, 2013·Periodontology 2000·Adolfo ContrerasJørgen Slots
Mar 21, 2007·Biochemical and Biophysical Research Communications·Hye-Ra LeeJin-Hyun Ahn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.