Absence of Integrase Strand Transfer Inhibitor Associated Resistance in Antiretroviral Therapy Naïve and Experienced Individuals from Western India

AIDS Research and Human Retroviruses
Santosh KaradeVangal Krishnaswamy Sashindran

Abstract

The Indian national AIDS control program heavily relies on low cost nucleoside reverse transcriptase inhibitor (NRTI) and non-nucleoside reverse transcriptase inhibitor (NNRTI). With global increase in resistance to these, alternative antiretroviral combinations need to be explored. Owing to higher potency, better efficacy and tolerability, recently WHO recommended integrase strand transfer inhibitor (INSTI) based first-line antiretroviral therapy (ART). There is lack of INSTI resistance surveillance data from India. Thus, there is a need to analyze integrase (IN) gene from primarily HIV-1 subtype C infected Indian population, before widespread introduction of INSTI in first-line ART. Plasma samples were collected from INSTI naïve individuals reporting to ART centre of Pune, India. RNA was extracted and IN gene was amplified by nested polymerase chain reaction (PCR) using prior published primers. PCR product of 867 bp was bi-directionally sequenced and resistance associated mutation were analyzed using Stanford University HIV drug resistance algorithm. A total of 58 HIV-1 sequences from 62 INSTI naïve individuals were successfully genotyped. Of these 58, 40 were ART naïve, newly diagnosed and remaining individuals were on NRTI,...Continue Reading

References

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Sep 8, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nagalingeswaran KumarasamyConstance A Benson

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Citations

Jul 31, 2020·The Journal of Antimicrobial Chemotherapy·Jean L MbisaAnna Maria Geretti

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Datasets Mentioned

BETA
MK076742
MK076799

Methods Mentioned

BETA
electrophoresis

Software Mentioned

jpHMM
SeqScape
HIVdb

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