Absence of laminin deposition in breast cancer and metastases except to the brain

Pathology, Research and Practice
V CastronovoJ M Foidart

Abstract

Laminin, a major glycoprotein of basement membrane has been found to play significant roles during invasion and metastases. In this study, we have examined the distribution of laminin in several human brain carcinoma metastases, human breast cancers, skin and lymph node metastases of breast cancer as well as in an in vitro and an in vivo model of invasion. A laminin accumulation was demonstrated a) at the border between human metastatic carcinoma cells and surrounding neural tissue; b) at the invasive edge between MO4 cells (a highly malignant cell line which synthesizes large amounts of laminin) and host tissues of syngenic mice; c) at the front of invasion between MO4 cells and precultured heart fragments in an in vitro model of invasion. Laminin, but not type IV collagen, promoted attachment of MO4 cells. This attachment was inhibited by preincubation of laminin matrix support with (+)-catechin, a flavonoid which also prevented invasion of the precultured heart fragment in vitro. Our data demonstrate that laminin accumulates between malignant cells and host tissue in human brain metastases and in an in vitro and an in vivo model of invasion. In these later models, accumulation of laminin is the consequence, at least in part,...Continue Reading

References

Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Apr 1, 1984·Clinical & Experimental Metastasis·M M MareelH Van Rooy
Apr 1, 1984·Clinical & Experimental Metastasis·M E BrackeM M Mareel

Related Concepts

Carcinoma, Cribriform
Brain Tumor, Recurrent
Mammary Neoplasms, Human
Cell Line, Transformed
SDS-PAGE
Immunofluorescence Assay
Peroxidase-Antiperoxidase Complex Technique
Merosin
Neoplasm Invasiveness
Tissue Specificity

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