Absence of lipopolysaccharide-induced inhibition of neutrophil apoptosis in patients with diabetes

Archives of Surgery
S D TennenbergA Dwivedi


Patients with diabetes mellitus are considered to be at increased risk for infections and often experience more serious and prolonged infections. Defects in neutrophil microbicidal function have been identified in diabetic patients and are believed to contribute to this problem. Neutrophil apoptosis or programmed cell death regulates functional longevity, and is an integral component of inflammation and its resolution. It remains unknown whether neutrophils from diabetic patients demonstrate defects in apoptosis. Neutrophils from diabetic patients have defects in spontaneous and/or endotoxin (lipopolysaccharide [LPS])-induced apoptosis. Peripheral venous blood samples were collected from healthy volunteers and insulin-dependent diabetic patients to harvest neutrophils for in vitro study. Neutrophils were cultured for 0 and 24 hours in the absence and presence of LPS. Normal neutrophils were also cultured under conditions of normal or high glucose concentration. Percentage of apoptotic cells was determined by flow cytometry. Normal neutrophils undergo significant apoptosis after 24 hours, and the percentage of apoptotic cells is reduced in the presence of LPS. Diabetic neutrophils undergo normal spontaneous apoptosis, but do not...Continue Reading


Jan 31, 2014·Indian Journal of Clinical Biochemistry : IJCB·Saba KhanPradyumna K Mishra
Apr 28, 2004·Journal of General Internal Medicine·Nicolas N AbourizkParveen K Verma
Oct 29, 2008·Infection and Immunity·Sujin ChanchamroenGanjana Lertmemongkolchai
Feb 8, 2013·BMC Veterinary Research·Anelise M BoscoPaulo C Ciarlini
Apr 25, 2006·Surgical Infections·Matthias TurinaHiram C Polk
Jan 15, 2008·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Jerard Kneifati-HayekRita Bellevue
Apr 11, 2009·Apoptosis : an International Journal on Programmed Cell Death·Aidan RyanFionnuala B Hickey
Dec 5, 2013·International Journal of Evidence-based Healthcare·Brian CheeP Mark Bartold
Oct 21, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jean-Francois DhainautDavid R Nelson
Dec 15, 2000·European Journal of Clinical Investigation·S E GeerlingsA I Hoepelman
Oct 4, 2002·The American Journal of Medicine·Lee N MetchickSection of Endocrinology, Department of Medicine, Yale Universtiy School of Medicine, New Haven, Connecticut
Nov 19, 2013·Tropical Medicine & International Health : TM & IH·Susan P Fisher-HochJoseph B McCormick
Jun 12, 2004·The Journal of Investigative Dermatology·Ghada NaguibDana T Graves
Sep 11, 2007·Infectious Disease Clinics of North America·Smita GuptaNancy Khardori
Apr 19, 2003·International Endodontic Journal·P G SatchellD E Witherspoon
Nov 15, 2006·Clinical and Experimental Immunology·Elaine HatanakaA Campa
Apr 12, 2013·Periodontology 2000·Robert J Genco, Wenche S Borgnakke
Sep 7, 2005·Life Sciences·Tatiana Carolina Alba-LoureiroP Sannomiya
Jun 13, 2003·Endocrinology and Metabolism Clinics of North America·Babette Carlson Glister, Robert A Vigersky
Feb 13, 2004·The Journal of Veterinary Medical Science·Junichi SanoAtsuhiko Hasegawa
May 18, 2016·Journal of Diabetes Investigation·Juan HuangZhiguang Zhou
Jun 20, 2003·Journal of Endodontics·I B Bender, A B Bender
Jun 30, 2007·Current Opinion in Critical Care·Juan Jose Blondet, Greg J Beilman
Jul 9, 2005·Critical Care Medicine·Matthias TurinaHiram C Polk
Nov 28, 2013·Current Opinion in Hematology·Manuela Battaglia
Feb 26, 2008·FEMS Immunology and Medical Microbiology·Tigran K DavtyanSamvel A Avetisyan
Nov 18, 2018·Frontiers in Physiology·Anamika Bajpai, Douglas G Tilley
Feb 7, 2017·Frontiers in Immunology·Wilson Mitsuo Tatagiba KuwabaraTatiana Carolina Alba-Loureiro
Dec 13, 2019·Mediators of Inflammation·Leandro BorgesElaine Hatanaka

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Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis