Absence of low molecular weight DNA polymerase activity from the nuclei of Amoeba discoides

Experientia
G F Abbott, S E Hawkins

Abstract

Amoeba discoides nuclear protein partially purified by passage through Sephadex G-200 showed 3 high-mol.-wt. DNA polymerase activities which eluted in and just following the void volume. No low-mol.-wt (45,000 daltons) DNA polymerase beta activity was detected. Nuclear protein layered on 5--20% sucrose gradients also showed an absence of low-mol.-wt DNA polymerase beta. The void volume enzyme showed deoxyribonuclease activity, but no low-mol.-wt nuclease activity was detected.

References

Jun 1, 1975·Cell·A Weissbach
Mar 19, 1976·Science·L M Chang
Apr 2, 1976·Biochimica Et Biophysica Acta·L W LoomisL M Chang
Jan 20, 1975·Biochimica Et Biophysica Acta·A Tait, D J Cummings
Jan 1, 1974·Methods in Enzymology·B S Fansler, L A Loeb

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Citations

Dec 20, 1979·Nucleic Acids Research·Y FurukawaM Kohno

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