Absence of membrane phosphatidylcholine does not affect virulence and stress tolerance phenotypes in the opportunistic pathogen Pseudomonas aeruginosa.

PloS One
Adel A MalekDeborah A Hogan

Abstract

During growth in presence of choline, both laboratory and clinical Pseudomonas aeruginosa strains synthesize phosphatidylcholine (PC), and PC makes up ∼4% of the total membrane phospholipid content. In all the strains tested, PC synthesis occurred only when choline is provided exogenously. Mutants defective in synthesis of PC were generated in the strain backgrounds PAO1 and PA14. Minimum inhibitory concentration studies testing sensitivity of PC-deficient strains towards various antibiotics and cationic antimicrobial peptides revealed no differences as compared to wild-type strains. Mutants incapable of synthesizing PC were also found to be unaffected in motility and biofilm formation on abiotic surfaces, colonization of biotic surfaces and virulence in a mouse infection model. A global phenotypic microarray was further used to identify conditions wherein membrane PC may play a role of in P. aeruginosa. No culture conditions were identified wherein wild-type and PC-deficient mutants showed phenotypic differences. Membrane PC may serve a highly specific role during P. aeruginosa interactions with its eukaryotic hosts based on all the clinical strains tested retaining the ability to synthesize it during availability of choline.

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Citations

Jan 29, 2013·Applied and Environmental Microbiology·Matthew J Wargo
May 14, 2014·International Journal of Molecular Sciences·Marta Palusinska-SzyszWitold Danikiewicz
Dec 18, 2013·Molecular Microbiology·Roman MoserFranz Narberhaus
Apr 12, 2014·Frontiers in Plant Science·Meriyem AktasFranz Narberhaus
Apr 23, 2013·Comparative Immunology, Microbiology and Infectious Diseases·Maria Andonova, Valentina Urumova
Jul 23, 2020·BioMed Research International·Moad KhalifaWei Cun See Too
Sep 11, 2019·Journal of Bacteriology·Luke R JoyceKelli L Palmer
Nov 19, 2015·European Journal of Mass Spectrometry·Yuqian WangXiaoyuan Wang
Aug 10, 2021·Frontiers in Plant Science·Otto GeigerIsabel M López-Lara

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Methods Mentioned

BETA
motility assay
motility assays
PCR
bronchoalveolar lavage

Software Mentioned

Prism
Omnilog®
GraphPad

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